南方科技大学知识苑(SUSTech KC): Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H<sub>2</sub>O<sub>2</sub>-induced granulosa cells apoptosis via USP48 protein regulation

题名	Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation
作者	Chen, Jun1,2 ; Lin, Chuhua 1,2; Huang, Xiurong 3,4; Bian, Wei 1,2
通讯作者	Bian, Wei
发表日期	2024-01-20
DOI	10.1186/s12906-024-04346-z
发表期刊	BMC COMPLEMENTARY MEDICINE AND THERAPIES 影响因子和分区
EISSN	2662-7671
卷号	24 期号:1
摘要	Background Oxidative stress and inflammation can lead to apoptosis of ovarian granulosa cells (GCs), resulting in ovulation disorders and infertility. Baicalin (BAI) promotes cell proliferation and reduces inflammation and oxidative stress. However, the mechanisms by which BAI treatment affects oxidative stress and inflammation in GCs remain incompletely understood.Methods KGN cells were treated with hydrogen peroxide (H2O2) to analyze the effect of oxidative stress on GCs in vitro. Subsequently, H2O2-stimulated KGN cells were treated with BAI. The levels of GSH-Px, CAT, and SOD were measured using an activity assay kit. The levels of MDA, IL-1 beta, IL-6, IL-8, and TNF-alpha were measured by ELISA. Proliferation, apoptosis, and mRNA and protein levels were measured using the CCK8, flow cytometry, qRT-PCR, and western blotting.Results H2O2 treatment inhibited KGN cell proliferation and promoted apoptosis, accompanied by increased oxidative stress and inflammation. BAI promoted proliferation, inhibited apoptosis, and reduced oxidative stress and inflammation in H2O2-stimulated KGN cells. BAI treatment promoted USP48 protein expression, and USP48 knockdown abrogated the protective effects of BAI, indicating that USP48 is a downstream mediator of BAI.Conclusion BAI treatment enhanced cell proliferation and ameliorated oxidative stress and inflammation by enhancing USP48 protein expression. BAI, which is used clinically and as a dietary supplement, may alleviate oxidative stress-induced GC injury and ovarian disorders.
关键词	USP48 Baicalin Oxidative stress Inflammation Ovarian granulosa cell
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
WOS研究方向	Integrative & Complementary Medicine
WOS类目	Integrative & Complementary Medicine
WOS记录号	WOS:001146217200001
出版者	BMC
来源库	Web of Science
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/789365
专题	南方科技大学第一附属医院
作者单位	1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Tradit Chinese Med, Shenzhen 518020, Peoples R China 2.Southern Univ Sci & Technol, Affiliated Hosp 1, 1017,Dongmen North Rd, Shenzhen 518020, Peoples R China 3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Rehabil Med, Shenzhen 518020, Peoples R China 4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Chen, Jun,Lin, Chuhua,Huang, Xiurong,et al. Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation[J]. BMC COMPLEMENTARY MEDICINE AND THERAPIES,2024,24(1).
APA	Chen, Jun,Lin, Chuhua,Huang, Xiurong,&Bian, Wei.(2024).Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation.BMC COMPLEMENTARY MEDICINE AND THERAPIES,24(1).
MLA	Chen, Jun,et al."Baicalin enhances proliferation and reduces inflammatory-oxidative stress effect in H₂O₂-induced granulosa cells apoptosis via USP48 protein regulation".BMC COMPLEMENTARY MEDICINE AND THERAPIES 24.1(2024).