Classical famous prescription of Jichuan decoction improved loperamide-induced slow transit constipation in rats through the cAMP/PKA/AQPs signaling pathway and maintained inflammatory/intestinal flora homeostasis

Lin, Lina1,2; Jiang, Yuanyuan3,4; Lin, Pengfei2,5; Ge, Lanlan3,4,6; Wan, Haoqiang3,4,6; Dai, Shuwen2; Zhang, Runjing2,7; Yao, Jie3; Zeng, Xiaobin3,4,6,8; Peng, Ying1

2024-01-15

10.1016/j.heliyon.2023.e21870

HELIYON   影响因子和分区

2405-8440

Introduction: Jichuan decoction (JCD) is a well-known traditional Chinese medicinal formula that moistens the intestines and is widely used for the treatment of constipation in China. However, its effects and mechanisms in alleviating slow transit constipation (STC) in vivo remain unclear. We attempted to demonstrate the effect of JCD, with and without essential oil (VO), on intestinal transit and its underlying molecular mechanisms in rats with loperamide-induced STC. Materials and methods: Water consumption, body weight, fecal water content, time to first melena excretion, and intestinal transit ratio of the animals were measured. 5-Hydroxytryptamine (5-HT), substance P (SP), vasoactive intestinal peptide (VIP), and interleukin-6 (IL-6) levels in the sera of rats were evaluated using ELISA. Hematoxylin and eosin and Periodic Acid-Schiff staining were used to determine intestinal tissue histology, while quantitative real-time PCR, western blotting, and immunohistochemical analysis were used to assess the relative expression levels of cAMP/ PKA/AQPs pathway- and inflammation-related proteins. 16 S rDNA sequence analysis of rat feces was used to determine the diversity and abundance of the intestinal flora. Results: The JCD groups showed reduced time to first melena excretion and expression of VIP and IL-6. The JCD groups, specifically JCD + VO groups, showed increased fecal water content, intestinal transit rate, and SP expression. Further, these groups showed improved histological characteristics of the colon, with no significant change in the index of immune organs or morphological characteristics of other organs. In addition, a significant decrease in the activation of the cAMP/PKA/AQPs signaling pathway in the colon tissue was observed in these groups, specifically the JCD + VO groups. Moreover, treatment with JCD, with or without VO, downregulated the expression of inflammatory factors and enriched the diversity of intestinal flora as evidenced by polymorphism analysis and the contents of Bacteroides, Lactobacillus, and Erysipelas, with the JCD + VO groups showing better therapeutic outcomes. Conclusion: JCD improved loperamide-induced STC, and co-administration with VO exhibited better activity than sole JCD therapy. JCD may improve STC by inhibiting the cAMP/PKA/AQPs signaling pathway and maintaining inflammatory/intestinal flora homeostasis.

Jichuan decoction Slow transit constipation cAMP/PKA/AQPs pathway Inflammation Intestinal flora homeostasis

SCI

英语

通讯

Natural Science Foundation of Guangdong Province[2021A1515220185]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:001141156200001

CELL PRESS

Web of Science

1.Shenyang Pharmaceut Univ, Wuya Coll Innovat, Shenyang 110016, Liaoning, Peoples R China
2.China Resources Sanjiu Med & Pharmaceut Co Ltd, Shenzhen 518000, Guangdong, Peoples R China
3.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Cent Lab Longhua Branch,Clin Med, Shenzhen 518020, Guangdong, Peoples R China
4.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Dept Infect Dis,Clin Med Coll 2, Shenzhen 518020, Guangdong, Peoples R China
5.Shenzhen Tradit Chinese Med Mfg Innovat Ctr Co Ltd, Shenzhen 518110, Guangdong, Peoples R China
6.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Pathol,Affiliated Hosp 1,Clin Med Coll 2,Long, Shenzhen 518020, Guangdong, Peoples R China
7.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, Shanghai 201203, Peoples R China
8.Shenzhen Univ, Med Sch, Guangdong Prov Key Lab Reg Immun & Dis, Shenzhen 518037, Guangdong, Peoples R China

Lin, Lina,Jiang, Yuanyuan,Lin, Pengfei,et al. Classical famous prescription of Jichuan decoction improved loperamide-induced slow transit constipation in rats through the cAMP/PKA/AQPs signaling pathway and maintained inflammatory/intestinal flora homeostasis[J]. HELIYON,2024,10(1).

Lin, Lina.,Jiang, Yuanyuan.,Lin, Pengfei.,Ge, Lanlan.,Wan, Haoqiang.,...&Peng, Ying.(2024).Classical famous prescription of Jichuan decoction improved loperamide-induced slow transit constipation in rats through the cAMP/PKA/AQPs signaling pathway and maintained inflammatory/intestinal flora homeostasis.HELIYON,10(1).

Lin, Lina,et al."Classical famous prescription of Jichuan decoction improved loperamide-induced slow transit constipation in rats through the cAMP/PKA/AQPs signaling pathway and maintained inflammatory/intestinal flora homeostasis".HELIYON 10.1(2024).