Human milk exosome-derived circDNAJB6 improves bronchopulmonary dysplasia model by promoting DNAJB6 gene transcription

Li, Yubai1; Yu, Boshi1; Li, Huimin2; Hou, Weiwei4; Yin, Jing2; Zhou, Yahui3; Yu, Zhangbin1

2024-04-01

10.1007/s10863-024-10002-5

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES   影响因子和分区

0145-479X

1573-6881

To verify the protective effect of circDNAJB6 on Bronchopulmonary dysplasia (BPD) cell and animal models and to explore the possible mechanism of its protective effect. The function of circDNAJB6 was investigated at the cell and animal levels. Nuclear and Cytoplasmic RNA extraction kits and fluorescence in situ hybridization (FISH) were used to explore the distribution of circDNAJB6 in cells, and the potential mechanism of circDNAJB6 was verified by q-PCR, luciferase assays and rescue experiments.CircDNAJB6 is abundant in breast milk exosomes. Overexpression of circDNAJB6 can ameliorate damage in BPD models caused by hyperoxia exposure in vivo and in vitro. Mechanistically, circDNAJB6 can target the downstream DNAJB6 gene and promote the transcription of DNAJB6, exertive a protective effect on the experimental BPD model. Our results showed that circDNAJB6 alleviated damage and inhibited the proliferation of alveolar epithelial cells in the BPD model by promoting transcription of parent gene DNAJB6. Human milk exosome-derived circDNAJB6 provides new directions for preventing and treating BPD.

Bronchopulmonary dysplasia Exosomes CircRNA Alveolar type II cell DNAJB6

SCI

英语

第一 ; 通讯

Biophysics ; Cell Biology

Biophysics ; Cell Biology

WOS:001145065900001

SPRINGER/PLENUM PUBLISHERS

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Div Neonatol,Dept Pediat,Clin Med Coll 2,Affiliate, Shenzhen 518020, Peoples R China
2.Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Womens Hosp, Dept Pediat, Nanjing 210004, Peoples R China
3.Jiangnan Univ, Wuxi Childrens Hosp, Dept Pediat, Div Neonatol,Childrens Hosp, Wuxi 214000, Peoples R China
4.Nanjing Med Univ, Clin Sch Med 1, Nanjing 210000, Peoples R China

Li, Yubai,Yu, Boshi,Li, Huimin,et al. Human milk exosome-derived circDNAJB6 improves bronchopulmonary dysplasia model by promoting DNAJB6 gene transcription[J]. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES,2024,56(2).

Li, Yubai.,Yu, Boshi.,Li, Huimin.,Hou, Weiwei.,Yin, Jing.,...&Yu, Zhangbin.(2024).Human milk exosome-derived circDNAJB6 improves bronchopulmonary dysplasia model by promoting DNAJB6 gene transcription.JOURNAL OF BIOENERGETICS AND BIOMEMBRANES,56(2).

Li, Yubai,et al."Human milk exosome-derived circDNAJB6 improves bronchopulmonary dysplasia model by promoting DNAJB6 gene transcription".JOURNAL OF BIOENERGETICS AND BIOMEMBRANES 56.2(2024).