An integrated analysis of bulk and single-cell sequencing data reveals that EMP1⁺/COL3A1⁺ fibroblasts contribute to the bone metastasis process in breast, prostate, and renal cancers

Du, Haoyuan1; Wang, Hua1; Luo, Yuwei2,3; Jiao, Yang4; Wu, Jiajun5; Dong, Shaowei5; Du, Dong6

2023-12-19

10.3389/fimmu.2023.1313536

FRONTIERS IN IMMUNOLOGY   影响因子和分区

1664-3224

Introduction: Bone metastasis (BoM) occurs when cancer cells spread from their primary sites to a bone. Currently, the mechanism underlying this metastasis process remains unclear.Methods: In this project, through an integrated analysis of bulk-sequencing and single-cell RNA transcriptomic data, we explored the BoM-related features in tumor microenvironments of different tumors.Results: We first identified 34 up-regulated genes during the BoM process in breast cancer, and further explored their expression status among different components in the tumor microenvironment (TME) of BoM samples. Enriched EMP1+ fibroblasts were found in BoM samples, and a COL3A1-ADGRG1 communication between these fibroblasts and cancer cells was identified which might facilitate the BoM process. Moreover, a significant correlation between EMP1 and COL3A1 was identified in these fibroblasts, confirming the potential connection of these genes during the BoM process. Furthermore, the existence of these EMP1+/COL3A1+ fibroblasts was also verified in prostate cancer and renal cancer BoM samples, suggesting the importance of these fibroblasts from a pan-cancer perspective.Discussion: This study is the first attempt to investigate the relationship between fibroblasts and BoM process across multi-tumor TMEs. Our findings contribute another perspective in the exploration of BoM mechanism while providing some potential targets for future treatments of tumor metastasis.

bone metastasis scRNAseq multi-tumor TMEs combined analysis EMP1+/COL3A1+fibroblasts

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[32000465]

Immunology

Immunology

WOS:001134350400001

FRONTIERS MEDIA SA

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Orthoped &, Shenzhen, Guangdong, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Dept Breast Surg, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Ultrasound, Shenzhen, Guangdong, Peoples R China
5.Shenzhen Childrens Hosp, Dept Pediat Res, Shenzhen, Guangdong, Peoples R China
6.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Hlth Manage, Shenzhen, Guangdong, Peoples R China

Du, Haoyuan,Wang, Hua,Luo, Yuwei,et al. An integrated analysis of bulk and single-cell sequencing data reveals that EMP1⁺/COL3A1⁺ fibroblasts contribute to the bone metastasis process in breast, prostate, and renal cancers[J]. FRONTIERS IN IMMUNOLOGY,2023,14.

Du, Haoyuan.,Wang, Hua.,Luo, Yuwei.,Jiao, Yang.,Wu, Jiajun.,...&Du, Dong.(2023).An integrated analysis of bulk and single-cell sequencing data reveals that EMP1⁺/COL3A1⁺ fibroblasts contribute to the bone metastasis process in breast, prostate, and renal cancers.FRONTIERS IN IMMUNOLOGY,14.

Du, Haoyuan,et al."An integrated analysis of bulk and single-cell sequencing data reveals that EMP1⁺/COL3A1⁺ fibroblasts contribute to the bone metastasis process in breast, prostate, and renal cancers".FRONTIERS IN IMMUNOLOGY 14(2023).