Effect of CeO2 Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023

Yu, Kaiqiang1,2,3,4; Zhao, Bixi1; Yan, Yuxi1; Yang, Qing1; Chen, Liming1; Xia, Yu1,3,4

2023-11-17

10.46234/ccdcw2023.194

CHINA CDC WEEKLY   影响因子和分区

2096-7071

2097-3101

Introduction: The use of reclaimed water (RW) for irrigation in agricultural practices raises concerns regarding the dissemination of antibiotic resistance genes (ARGs) from soils to edible crops. The effectiveness of nanoparticles (NPs) in reducing antibiotic resistance in vegetables irrigated with RW remains largely unexplored. Methods: To investigate the effects, we conducted pot experiments in which radishes were planted in soil amended with CeO2 NPs using various application techniques. The abundance of ARGs was characterized using high-throughput quantitative PCR (HT-qPCR). Concurrently, we utilized 16S ribosomal RNA (rRNA) gene sequencing to evaluate the microbial community structure of both the rhizosphere soil and the endophytic compartment within the radishes. Employing bioinformatics analysis, we probed the potential mechanisms by which NPs influence the resistome within the reclaimed water-soil-radish system.Results: Following the application of CeO2 NPs, there was a noticeable reduction in both the number and concentration of ARG genotypes in the rhizosphere soil, as well as within the radish. Concurrently, CeO2 NPs appeared to mitigate the propagation of ARGs within the reclaimed water-soil radish system. The ability of CeO2 NPs to modulate the resistome is linked to alterations in microbial community structure. Soil treatment with NPs emerged as the most effective strategy for curbing the spread of ARGs. Discussion: This finding provides a theoretical foundation for the development of nano-agricultural technologies aimed at controlling the proliferation of ARGs.

SCI ; SSCI

英语

第一 ; 通讯

National Key Research and Development Program of China[2021YFA1202500] ; National Natural Science Foundation of China["42107257","42007216"]

Public, Environmental & Occupational Health

Public, Environmental & Occupational Health

WOS:001123724700003

Chinese Center for Disease Control and Prevention

Web of Science

1.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen, Guangdong, Peoples R China
2.Ningxia Normal Univ, Sch Resource Environm & Life Sci, Guyuan, Gansu, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Key Lab Soil & Groundwater Pollut Contr, Shenzhen, Guangdong, Peoples R China

Yu, Kaiqiang,Zhao, Bixi,Yan, Yuxi,et al. Effect of CeO2 Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023[J]. CHINA CDC WEEKLY,2023,5(46).

Yu, Kaiqiang,Zhao, Bixi,Yan, Yuxi,Yang, Qing,Chen, Liming,&Xia, Yu.(2023).Effect of CeO2 Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023.CHINA CDC WEEKLY,5(46).

Yu, Kaiqiang,et al."Effect of CeO2 Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023".CHINA CDC WEEKLY 5.46(2023).