Circulating miRNAs act as potential biomarkers for asthma

Hu, Guang1,2; Du, Yujie1,2; Xie, Manying2,3; Chen, Rongchang2,4; Shi, Fei1,2

2023-12-19

10.3389/fimmu.2023.1296177

FRONTIERS IN IMMUNOLOGY   影响因子和分区

1664-3224

BackgroundIdentification of new clinical markers contributes to a better understanding of the pathogenesis of asthma. Considering the crucial role of LIGHT in asthma, it may become a potential target for asthma. The aim of current study was to determine if circulating microRNAs (miRNAs) targeting LIGHT may be used as diagnostic biomarkers to distinguish asthma.MethodsBlood serum from a cohort of 60 subjects, including 20 cases with mild asthma, 20 cases with moderate-to-severe asthma, and 20 healthy controls were included. Serum was analyzed for circulating miRNAs profiles through miRNAs microarray. Real Time PCR was conducted to verify the results of miRNA microarray. Correlations between circulating miRNAs targeting LIGHT and clinical characteristics were investigated.ResultsA total of 365 miRNAs were differentially expressed in asthma patients. Among them, miR-107 and miR-140-5p were found to target LIGHT, and varied in asthmatics. Additionally, miR-107 and miR-140-5p expressions were positively correlated with the absolute value of peripheral eosinophils. Finally, miR-140-5p and miR-107 were demonstrated to have good diagnostic efficacy for asthma (AUC= 0.8667 and 0.9400) with good sensitivity (0.8000 and 0.8667,respectively) and specificity (0.8667 and 0.867). Thus, circulating miRNAs expressed differentially between healthy control and asthma patients.ConclusionPlasma miR-140-5p and miR-107 can be used as diagnostic biomarkers to distinguish patients with asthma from healthy control, and may take part in asthma pathogenesis by negatively regulating LIGHT. Further research was needed to evaluate their roles as potential biomarkers in the diagnosis of asthma.

biomarker circulating miRNA asthma light diagnosis

SCI

英语

通讯

Basic and applied Basic Research Foundation of Guangdong Province, China[2022A1515220169] ; Natural Science Foundation of China[81300012] ; Appropriate Health Technology Promotion Project of Guangdong Province[:202006181142034974] ; Scientific Research and Cultivation Project of Shenzhen People's Hospital[SYKYPY201912]

Immunology

Immunology

WOS:001134028500001

FRONTIERS MEDIA SA

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Infect Dis, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Intervent Dept, Shenzhen, Guangdong, Peoples R China
4.Jinan Univ, Shenzhen Peoples Hosp, Inst Shenzhen Resp Dis, Key Lab Shenzhen Resp Dis,Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China

Hu, Guang,Du, Yujie,Xie, Manying,et al. Circulating miRNAs act as potential biomarkers for asthma[J]. FRONTIERS IN IMMUNOLOGY,2023,14.

Hu, Guang,Du, Yujie,Xie, Manying,Chen, Rongchang,&Shi, Fei.(2023).Circulating miRNAs act as potential biomarkers for asthma.FRONTIERS IN IMMUNOLOGY,14.

Hu, Guang,et al."Circulating miRNAs act as potential biomarkers for asthma".FRONTIERS IN IMMUNOLOGY 14(2023).