南方科技大学知识苑(SUSTech KC): Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin Î²1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer

题名	Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin Î²1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer
作者	Liu, Jiaxin 1,5; Tang, Lu 5; Chu, Wenzhu 6; Wei, Lanlan 2,3,4,5
通讯作者	Wei, Lanlan
发表日期	2023-11-01
DOI	10.1007/s10528-023-10568-6
发表期刊	BIOCHEMICAL GENETICS 影响因子和分区
ISSN	0006-2928
EISSN	1573-4927
摘要	The ectopic expression of cellular retinoic acid binding protein 2 (CRABP2) is associated with various tumorigenesis. However, the effects of CRABP2 on the progression of cervical cancer are still unclear. The current study aimed to investigate the role of CRABP2 in the malignant phenotypes of cervical cancer cells. CRABP2 was artificially regulated in CaSki, SiHa, and C-33A cells. CCK-8 assay and flow cytometry were used to assess the cell proliferation and apoptosis abilities, respectively. Wound healing assay and transwell assay were employed to measure the cell migration and invasion abilities, respectively. The results showed that CRABP2 was highly expressed in cervical carcinoma tissues and cell lines, and its high expression was associated with poor overall survival. Knockdown of CRABP2 promoted the cell apoptosis and inhibited cell proliferation, migration, and invasion in cervical carcinoma cells, whereas CRABP2 overexpression exhibited the opposite results. Mechanically, CRABP2 silencing suppressed the Integrin beta 1/FAK/ERK signaling via HuR. Treatment with siITGB1 or a FAK inhibitor PF-562271 or an ERK inhibitor FR180204 reversed the promoting effects of CRABP2 on cell proliferation, migration, and invasion. Moreover, the overexpression of CRABP2 reverted the HPV16 E6/E7 knockdown-induced inhibition of cell proliferation, migration, and invasion in cervical cancer cells. These results suggested that HPV16 E6/E7 promoted the malignant phenotypes of cervical cancer by upregulating the expression of CRABP2. In conclusion, CRABP2, upregulated by HPV E6/E7, promoted the progression of cervical cancer through activating the Integrin beta 1/FAK/ERK signaling pathway via HuR.
关键词	Cervical carcinoma Cellular retinoic acid binding protein 2 HPV16 E6/E7 Integrin beta 1/FAK/ERK signaling
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Shenzhen Longgang Fund for Science and Technology Project[LGKCYLWS2020101] ; Shenzhen Science and Technology RD Fund[JCYJ20210324131607019] ; College-level Scientific Research Program of Taizhou Polytechnic College[TZYKYZD-23-1] ; Social Development Project on Science and Technology Support Program of Taizhou[TS202320] ; National Science Fund for Distinguished Young Scholars[82102822]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001122475100003
出版者	SPRINGER/PLENUM PUBLISHERS
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/789418
专题	南方科技大学
作者单位	1.Taizhou Polytech Coll, Sch Med Technol, Taizhou 225300, Jiangsu, Peoples R China 2.Natl Clin Res Ctr Infect Dis, Shenzhen 518000, Guangdong, Peoples R China 3.Third Peoples Hosp Shenzhen, Inst Hepatol, Shenzhen 518000, Guangdong, Peoples R China 4.Southern Univ Sci & Technol, Hosp 2, Shenzhen 518000, Guangdong, Peoples R China 5.Harbin Med Univ, Dept Microbiol, Immun & Infect Pathogen Biol Key Lab Heilongjiang, Harbin 150081, Peoples R China 6.Mudanjiang Med Univ, Hongqi Hosp, Dept Dermatol, Mudanjiang 157011, Heilongjiang, Peoples R China
通讯作者单位	南方科技大学
推荐引用方式 GB/T 7714	Liu, Jiaxin,Tang, Lu,Chu, Wenzhu,et al. Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin Î²1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer[J]. BIOCHEMICAL GENETICS,2023.
APA	Liu, Jiaxin,Tang, Lu,Chu, Wenzhu,&Wei, Lanlan.(2023).Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin Î²1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer.BIOCHEMICAL GENETICS.
MLA	Liu, Jiaxin,et al."Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin Î²1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer".BIOCHEMICAL GENETICS (2023).