Perfluorooctane Sulfonamide Induced Autotoxic Effects on the Zebrafish Immune System

Chen, Honghong1,2,3; Zou, Yao4,5; Kang, Xinyuan3; Yang, Ge3; Yang, Xin6; Yao, Yingying7; Magnuson, Jason T.8; Cao, Xinde2; Qiu, Wenhui3; Xu, Elvis Genbo9; Zheng, Chunmiao1,3

2024-07-01

10.1021/acs.est.4c01153

ENVIRONMENTAL SCIENCE & TECHNOLOGY   影响因子和分区

0013-936X

1520-5851

Perfluorooctane sulfonamide (PFOSA) is an immediate perfluorooctanesulfonate (PFOS) precursor (PreFOS). Previous studies have shown PFOSA to induce stronger toxic responses compared to other perfluorinated compounds (PFCs). However, the specific nature of PFOSA-induced toxicity, whether autonomous or mediated by its metabolite PFOS, has not been fully elucidated. This study systematically investigates the immunomodulatory effects of PFOSA and PFOS in zebrafish (Danio rerio). Exposure to PFOSA compromised the zebrafish's ability to defend against pathogenic infections, as evidenced by increased bacterial adhesion to their skin and reduced levels of the biocidal protein lysozyme (LYSO). Moreover, PFOSA exposure was associated with disruptions in inflammatory markers and immune indicators, along with a decrease in immune cell counts. The findings from this study suggest that the immunotoxicity effects of PFOSA are primarily due to its own toxicity rather than its metabolite PFOS. This conclusion was supported by dose-dependent responses, the severity of observed effects, and multivariate analysis. In addition, our experiments using NF-kappa B-morpholino knock-down techniques further confirmed the role of the Nuclear factor-kappa appa B pathway in mediating PFOSA-induced immunotoxicity. In conclusion, this study reveals that PFOSA impairs the immune system in zebrafish through an autotoxic mechanism, providing valuable insights for assessing the ecological risks of PFOSA.

PFOSA PreFOS Danio rerio immunotoxicity NF-kappa B pathway

SCI ; EI

英语

通讯

National Natural Science Foundation of China["42322707","42077223"] ; Guangdong-Hong Kong Joint Laboratory for Soil and Groundwater Pollution Control[2023B1212120001] ; Shenzhen Key Laboratory of Precision Measurement and Early Warning Technology for Urban Environmental Health Risks[ZDSYS20220606100604008] ; Shenzhen Key Laboratory of Soil and Groundwater Pollution Control[ZDSY20150831141712549] ; Shenzhen Science and Technology Innovation Committee["KCXFZ20201221173410029","20220814233432001"] ; Ningbo Public Welfare Technology Plan Project[2021S101] ; Ningbo key research and development plan and "reveal the list" project[2023Z175] ; Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control[2023B1212060002] ; Danmarks Frie Forskningsfond[0165-00056B]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:001266842700001

AMER CHEMICAL SOC

ENVIRONMENT/ECOLOGY

Web of Science

1.Eastern Inst Technol, Eastern Inst Adv Study, Ningbo 315200, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut, Shenzhen 518055, Peoples R China
4.South China Univ Technol, Sch Environm & Energy, Guangzhou 510006, Peoples R China
5.Guangdong Soc Environm Sci, Guangzhou 510045, Peoples R China
6.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen Key Lab Precis Measurement & Early Warni, Shenzhen 518055, Peoples R China
7.Xi An Jiao Tong Univ, Inst Global Environm Change, Xian 710049, Peoples R China
8.US Geol Survey, Columbia Environm Res Ctr, Columbia, MO 65201 USA
9.Univ Southern Denmark, Dept Biol, DK-5230 Odense, Denmark

Chen, Honghong,Zou, Yao,Kang, Xinyuan,et al. Perfluorooctane Sulfonamide Induced Autotoxic Effects on the Zebrafish Immune System[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2024.

Chen, Honghong.,Zou, Yao.,Kang, Xinyuan.,Yang, Ge.,Yang, Xin.,...&Zheng, Chunmiao.(2024).Perfluorooctane Sulfonamide Induced Autotoxic Effects on the Zebrafish Immune System.ENVIRONMENTAL SCIENCE & TECHNOLOGY.

Chen, Honghong,et al."Perfluorooctane Sulfonamide Induced Autotoxic Effects on the Zebrafish Immune System".ENVIRONMENTAL SCIENCE & TECHNOLOGY (2024).