Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients

Yang, Zhiqian1,2; Chen, Liangmei2; Huang, Yingxin2,6; Dong, Jingjing1,2; Yan, Qiang3; Li, Ya1; Qiu, Jing1; Li, Haitao1; Zhao, Da5; Liu, Fanna2; Tang, Donge1; Dai, Yong4,5

2024-08-01

10.1016/j.jchromb.2024.124231

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES   影响因子和分区

1570-0232

1873-376X

Diabetic nephropathy (DN) remains the primary cause of end-stage renal disease (ESRD), warranting equal attention and separate analysis of glomerular, tubular, and interstitial lesions in its diagnosis and intervention. This study aims to identify the specific proteomics characteristics of DN, and assess changes in the biological processes associated with DN. 5 patients with DN and 5 healthy kidney transplant donor control individuals were selected for analysis. The proteomic characteristics of glomeruli, renal tubules, and renal interstitial tissue obtained through laser capture microscopy (LCM) were studied using high-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS). Significantly, the expression of multiple heat shock proteins (HSPs), tubulins, and heterogeneous nuclear ribonucleoproteins (hnRNPs) in glomeruli and tubules was significantly reduced. Differentially expressed proteins (DEPs) in the glomerulus showed significant enrichment in pathways related to cell junctions and cell movement, including the regulation of actin cytoskeleton and tight junction. DEPs in renal tubules were significantly enriched in glucose metabolism-related pathways, such as glucose metabolism, glycolysis/gluconeogenesis, and the citric acid cycle. Moreover, the glycolysis/gluconeogenesis pathway was a co-enrichment pathway in both DN glomeruli and tubules. Notably, ACTB emerged as the most crucial protein in the protein -protein interaction (PPI) analysis of DEPs in both glomeruli and renal tubules. In this study, we delve into the unique proteomic characteristics of each sub-region of renal tissue. This enhances our understanding of the potential pathophysiological changes in DN, particularly the potential involvement of glycolysis metabolic disorder, glomerular cytoskeleton and cell junctions. These insights are crucial for further research into the identification of disease biomarkers and the pathogenesis of DN.

Diabetic nephropathy Laser capture microscopy Proteomics Glucose metabolism Cytoskeleton

SCI ; EI

英语

通讯

Shenzhen Science and Technology Program["JCYJ20210324113013035","JCYJ20210324113214039"] ; Postdoctoral Innovation Practice Base, Shenzhen Polytechnic[6020330001K] ; Special Project in Key Fields of Ordinary Universities in Guangdong Province[2021ZDZX2042] ; Guangzhou Science and Technology Plan[a01219] ; Guangzhou Science and Technology Bureau and Universities[202201020080] ; Fundamental Research Funds for the Central Universities[21622306] ; Basic and Applied Basic Research Project of Guangzhou[2023A04J1911]

Biochemistry & Molecular Biology ; Chemistry

Biochemical Research Methods ; Chemistry, Analytical

WOS:001270954200001

ELSEVIER

20242816692238

Cell membranes ; Diagnosis ; Diseases ; Glucose ; High performance liquid chromatography ; Mass spectrometry ; Metabolism ; Molecular biology ; Pathology ; Tissue

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Chemistry:801 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Jinan Univ, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Shenzhen Peoples Hosp,Shenzhen Clin Res Ctr Geriat, Shenzhen Engn Res Ctr Autoimmune Dis,Clin Med Coll, Shenzhen 518020, Peoples R China
2.Jinan Univ, Affiliated Hosp 1, Dept Nephrol, Guangzhou 510632, Peoples R China
3.924 Hosp, Dept Organ Transplantat, Guilin 541002, Peoples R China
4.Southern Univ Sci & Technol, Taizhou Res Inst, Comprehens Hlth Ind Res Ctr, Taizhou 317000, Peoples R China
5.Anhui Univ Sci & Technol, Affiliated Hosp 1, Sch Med, Huainan 232001, Anhui, Peoples R China
6.Xiaolan People?s Hosp Zhongshan, Dept Nephrol, Zhongshan 528400, Peoples R China

Yang, Zhiqian,Chen, Liangmei,Huang, Yingxin,et al. Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2024,1243.

Yang, Zhiqian.,Chen, Liangmei.,Huang, Yingxin.,Dong, Jingjing.,Yan, Qiang.,...&Dai, Yong.(2024).Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1243.

Yang, Zhiqian,et al."Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1243(2024).