The expression and clinical significance of CFAP65 in colon cancer

Li, Yunze1,2; Ran, Dongmei3,4; Basnet, Shiva1,2; Zhang, Buzhe1,2; Pei, Hongjing1,2; Dan, Chenchen1,2; Zhang, Zixuan1,2; Zhang, Liang5; Lu, Tianyu5; Peng, Yifan2,6; Du, Changzheng1,2,4,7,8

2024-07-11

10.1186/s12876-024-03317-5

BMC GASTROENTEROLOGY   影响因子和分区

1471-230X

BackgroundCFAP65 (cilia and flagella associated protein 65) is a fundamental protein in the development and formation of ciliated flagella, but few studies have focused on its role in cancer. This study aimed to investigate the prognostic significance of CFAP65 in colon cancer.MethodsThe functionally enriched genes related to CFAP65 were analyzed through the Gene Ontology (GO) database. Subsequently, CFAP65 expression levels in colon cancer were evaluated by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) and immunoblotting in 20 pairs of frozen samples, including tumors and their matched paratumor tissue. Furthermore, protein expression of CFAP65 in 189 colon cancer patients were assessed via immunohistochemical staining. The correlations between CFAP65 expression and clinical features as well as long-term survival were statistically analyzed.ResultsCFAP65-related genes are significantly enriched on cellular processes of cell motility, ion channels, and GTPase-associated signaling. The expression of CFAP65 was significantly higher in colon cancer tissue compared to paratumor tissue. The proportion of high expression and low expression of CFAP65 in the clinical samples of colon cancer were 61.9% and 38.1%, respectively, and its expression level was not associated with the clinical parameters including gender, age, tumor location, histological differentiation, tumor stage, vascular invasion and mismatch repair deficiency. The five-year disease-free survival rate of the patients with CFAP65 low expression tumors was significantly lower than that those with high expression tumors (56.9% vs. 72.6%, P = 0.03), but the overall survival rate has no significant difference (69% vs. 78.6%, P = 0.171). The cox hazard regression analysis model showed that CFAP65 expression, tumor stage and tumor location were independent prognostic factors.ConclusionsIn conclusion, we demonstrate CFAP65 is a potential predictive marker for tumor progression in colon cancer.

CFAP65 Colon cancer Prognosis Pathology Surgery

SCI

英语

通讯

Gastroenterology & Hepatology

Gastroenterology & Hepatology

WOS:001269602700001

BMC

CLINICAL MEDICINE

Web of Science

1.Peking Univ Canc Hosp & Inst, Gastrointestinal Canc Ctr, Key Lab Carcinogenesis & Translat Res, Dept Unit 3,Minist Educ, 52 Fucheng Rd, Beijing 100142, Peoples R China
2.Peking Univ Canc Hosp & Inst, Gastrointestinal Canc Ctr, Ostomy Serv, 52 Fucheng Rd, Beijing 100142, Peoples R China
3.Southern Univ Sci & Technol Hosp, Dept Pathol, Shenzhen 518055, Guangdong, Peoples R China
4.Southern Univ Sci & Technol Hosp, Digest Tumor Ctr, Shenzhen 518055, Guangdong, Peoples R China
5.Southern Univ Sci & Technol Hosp, Dept Gastroenterol, Shenzhen 518055, Guangdong, Peoples R China
6.Peking Univ Canc Hosp & Inst, Gastrointestinal Canc Ctr, Dept Unit 3, Beijing 100142, Peoples R China
7.Beijing Tsinghua Changgung Hosp, 168 Litang Rd, Beijing 102218, Peoples R China
8.Tsinghua Univ, Sch Med, 168 Litang Rd, Beijing 102218, Peoples R China

Li, Yunze,Ran, Dongmei,Basnet, Shiva,et al. The expression and clinical significance of CFAP65 in colon cancer[J]. BMC GASTROENTEROLOGY,2024,24(1).

Li, Yunze.,Ran, Dongmei.,Basnet, Shiva.,Zhang, Buzhe.,Pei, Hongjing.,...&Du, Changzheng.(2024).The expression and clinical significance of CFAP65 in colon cancer.BMC GASTROENTEROLOGY,24(1).

Li, Yunze,et al."The expression and clinical significance of CFAP65 in colon cancer".BMC GASTROENTEROLOGY 24.1(2024).