Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing

Zhang, Qiongdi1; Hao, Yuxuan1; Zeng, Tonghua1; Shu, Weiliang1; Xue, Pan1,2; Li, Yang1; Huang, Chi1; Ouyang, Liwei1; Zou, Xuming3,4; Zhao, Zhen1; Wang, Jiahong1; Yu, Xue-Feng1,5; Zhou, Wenhua1,5

2024-07-01

10.1002/advs.202401796

ADVANCED SCIENCE   影响因子和分区

2198-3844

["Graphene field-effect transistors (GFETs) are widely used in biosensing due to their excellent properties in biomolecular signal amplification, exhibiting great potential for high-sensitivity and point-of-care testing in clinical diagnosis. However, difficulties in complicated fabrication steps are the main limitations for the further studies and applications of GFETs. In this study, a modular fabrication technique is introduced to construct microfluidic GFET biosensors within 3 independent steps. The low-melting metal electrodes and intricate flow channels are incorporated to maintain the structural integrity of graphene and facilitate subsequent sensing operations. The as-fabricated GFET biosensor demonstrates excellent long-term stability, and performs effectively in various ion environments. It also exhibits high sensitivity and selectivity for detecting single-stranded nucleic acids at a 10 fm concentration. Furthermore, when combined with the CRISPR/Cas12a system, it facilitates amplification-free and rapid detection of nucleic acids at a concentration of 1 fm. Thus, it is believed that this modular-fabricated microfluidic GFET may shed light on further development of FET-based biosensors in various applications.","A modular fabrication strategy of microfluidic graphene FET biosensor for nucleic acids detection is designed based on PDMS technique and low-melting metal electrodes. The device is fabricated through three simple and independent steps within 1.5 hours. The as-fabricated biosensor exhibits long-term stability and good performances in various ionic environments, and enables reliable detection of nucleic acids down to fm range. image"]

biosensor CRISPR/Cas12a graphene field-effect transistor microfluidic modular fabrication

SCI ; EI

英语

其他

Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, National Key R&D Program of China[2023YFA0915600] ; National Natural Science Foundation of China["32271519","32101207"] ; Basic and Applied Basic Research Foundation of Guangdong Province["2020B1515120040","2022B1515020029"] ; Science and Technology Planning Project of Guangdong Province of China[2021A0505110012] ; Shenzhen Science and Technology Program["JCYJ20200109115408041","JCYJ20220818101413029"] ; Shenzhen Science and Technology Research Funding[JSGG20191115141601721] ; Youth Innovation Promotion Association Chinese Academy of Sciences[2020354]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001274603400001

WILEY

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen 518055, Peoples R China
3.Hunan Univ, Sch Phys & Elect, Key Lab Micro Nano Optoelect Devices, Minist Educ, Changsha 410082, Peoples R China
4.Hunan Univ, Sch Phys & Elect, Hunan Prov Key Lab Low Dimens Struct Phys & Device, Changsha 410082, Peoples R China
5.Chinese Acad Sci, Key Lab Biomed Imaging Sci & Syst, Shenzhen 518055, Peoples R China

Zhang, Qiongdi,Hao, Yuxuan,Zeng, Tonghua,et al. Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing[J]. ADVANCED SCIENCE,2024.

Zhang, Qiongdi.,Hao, Yuxuan.,Zeng, Tonghua.,Shu, Weiliang.,Xue, Pan.,...&Zhou, Wenhua.(2024).Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing.ADVANCED SCIENCE.

Zhang, Qiongdi,et al."Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing".ADVANCED SCIENCE (2024).