DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA

Li, Sheng1; Yin, Haofan2,3; Zheng, Jiale4; Wan, Yunzhu1; Wang, Ke1; Yang, Chongguang5; Zhou, Jianhua1,6; Zhao, Meng4; Yuan, Xiaopeng2,3; Wang, Jiasi1,6

2024-07-01

10.1021/acssensors.4c01248

ACS SENSORS   影响因子和分区

2379-3694

Rapid and precise nucleic acid testing at the point-of-care (POC) is essential for effective screening and management of infectious diseases. Current polymerase-based molecular diagnostics often suffer from potential cross-contamination issues, particularly in POC settings. Here, we introduce DECODE, a contamination-free nucleic acid detection platform integrating digital microfluidics (DMF) for nucleic acid extraction and a digital CRISPR amplification-free assay for pathogen detection. The digital CRISPR assay demonstrates sensitivity, detecting target DNA and RNA in the reaction mixture at concentrations of 10 and 5 copies/mu L, respectively. Leveraging DMF-extracted samples enhances the performance of the digital CRISPR amplification-free assay. DECODE offers a sample-to-result workflow of 75 min using compact devices. Validation studies using clinical samples confirm DECODE's robust performance, achieving 100% sensitivity and specificity in detecting HPV18 from cervical epithelial cells and influenza A from nasal swabs. DECODE represents a versatile, contamination-free detection platform poised to enhance integrated public health surveillance efforts.

CRISPR digital detection point-of-care testing amplification-free polydisperse droplet

SCI ; EI

英语

通讯

National Key Research and Development Program of China["2023YFC2307305","2021YFF0703300"] ; Shenzhen Medical Research Fund[B2303003] ; Shenzhen Research Funding Program["JCYJ20220818102014028","RCBS20210609104339043"] ; National Natural Science Foundation of China[22174167] ; R&D Program of Guangzhou Laboratory[SRPG22-001] ; Guangdong Basic and Applied Basic Research[2024A1515011281]

Chemistry ; Science & Technology - Other Topics

Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology

WOS:001273654900001

AMER CHEMICAL SOC

Web of Science

1.Sun Yat Sen Univ, Sch Biomed Engn, Guangdong Prov Key Lab Sensor Technol & Biomed Ins, Shenzhen Campus, Shenzhen 518107, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Med Lab, Shenzhen 518020, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
4.Micronano Tech Ctr, Bioland Lab, Guangzhou 510000, Guangdong, Peoples R China
5.Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Shenzhen Campus, Shenzhen 518107, Guangdong, Peoples R China
6.Sun Yat Sen Univ, Sch Biomed Engn, Guangzhou 510275, Peoples R China

Li, Sheng,Yin, Haofan,Zheng, Jiale,et al. DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA[J]. ACS SENSORS,2024.

Li, Sheng.,Yin, Haofan.,Zheng, Jiale.,Wan, Yunzhu.,Wang, Ke.,...&Wang, Jiasi.(2024).DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA.ACS SENSORS.

Li, Sheng,et al."DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA".ACS SENSORS (2024).