Spatial Proteomic Profiling of Colorectal Cancer Revealed Its Tumor Microenvironment Heterogeneity

Xie, Lifen1,2,3; Kong, Qian3; Ai, Meiling1,2,3; He, An3; Yao, Bin3; Zhang, Luobin1; Zhang, Keren3; Zhu, Chaowei5; Li, Yangqiu4; Xia, Ligang5; Tian, Ruijun3; Xu, Ruilian1

2024-07-01

10.1021/acs.jproteome.3c00719

JOURNAL OF PROTEOME RESEARCH   影响因子和分区

1535-3893

1535-3907

Colorectal cancer is a predominant malignancy with a second mortality worldwide. Despite its prevalence, therapeutic options remain constrained and surgical operation is still the most useful therapy. In this regard, a comprehensive spatially resolved quantitative proteome atlas was constructed to explore the functional proteomic landscape of colorectal cancer. This strategy integrates histopathological analysis, laser capture microdissection, and proteomics. Spatial proteome profiling of 200 tissue section samples facilitated by the fully integrated sample preparation technology SISPROT enabled the identification of more than 4000 proteins on the Orbitrap Exploris 240 from 2 mm(2) x 10 mu m tissue sections. Compared with normal adjacent tissues, we identified a spectrum of cancer-associated proteins and dysregulated pathways across various regions of colorectal cancer including ascending colon, transverse colon, descending colon, sigmoid colon, and rectum. Additionally, we conducted proteomic analysis on tumoral epithelial cells and paracancerous epithelium from early to advanced stages in hallmark rectum cancer and sigmoid colon cancer. Bioinformatics analysis revealed functional proteins and cell-type signatures associated with different regions of colorectal tumors, suggesting potential clinical implications. Overall, this study provides a comprehensive spatially resolved functional proteome landscape of colorectal cancer, serving as a valuable resource for exploring potential biomarkers and therapeutic targets.

colorectal cancer heterogeneity spatial resolution cell type proteomics

SCI

英语

通讯

National Natural Science Foundation of China["32171433","22104047","22004049","81900034","22125403","22004056"] ; China State Key Basic Research Program Grants["2021YFA1301601","2020YFE0202200","2021YFA1302603","2022YFC3401104","2021YFA1301602"] ; Shenzhen Innovation of Science and Technology Commission["JSGGZD20220822095200001","JCYJ20200109141212325","JCYJ20210324120210029"]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:001273643500001

AMER CHEMICAL SOC

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Oncol, Shenzhen 518020, Peoples R China
2.Jinan Univ, Affiliated Hosp 1, Guangzhou 510630, Peoples R China
3.Southern Univ Sci & Technol, Dept Chem, Coll Sci, Shenzhen 518055, Peoples R China
4.Jinan Univ, Affiliated Hosp 1, Key Lab Regenerat Med, Minist Educ,Inst Hematol,Sch Med,Dept Hematol, Guangzhou 510632, Peoples R China
5.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Gastrointestinal Surg, Shenzhen 518020, Guangdong, Peoples R China

Xie, Lifen,Kong, Qian,Ai, Meiling,et al. Spatial Proteomic Profiling of Colorectal Cancer Revealed Its Tumor Microenvironment Heterogeneity[J]. JOURNAL OF PROTEOME RESEARCH,2024.

Xie, Lifen.,Kong, Qian.,Ai, Meiling.,He, An.,Yao, Bin.,...&Xu, Ruilian.(2024).Spatial Proteomic Profiling of Colorectal Cancer Revealed Its Tumor Microenvironment Heterogeneity.JOURNAL OF PROTEOME RESEARCH.

Xie, Lifen,et al."Spatial Proteomic Profiling of Colorectal Cancer Revealed Its Tumor Microenvironment Heterogeneity".JOURNAL OF PROTEOME RESEARCH (2024).