Identification of prostate cancer bone metastasis related genes and potential therapy targets by bioinformatics and in vitro experiments

Jiang, Haiyang1,2; Liu, Mingcheng3; Deng, Yingfei4; Zhang, Chongjian1; Dai, Longguo1; Zhu, Bingyu1; Ou, Yitian2; Zhu, Yong1; Hu, Chen1; Yang, Libo1; Li, Jun1; Bai, Yu1; Yang, Delin2

2024-08-01

10.1111/jcmm.18511

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE   影响因子和分区

1582-1838

1582-4934

The aetiology of bone metastasis in prostate cancer (PCa) remains unclear. This study aims to identify hub genes involved in this process. We utilized machine learning, GO, KEGG, GSEA, Single-cell analysis, ROC methods to identify hub genes for bone metastasis in PCa using the TCGA and GEO databases. Potential drugs targeting these genes were identified. We validated these results using 16 specimens from patients with PCa and analysed the relationship between the hub genes and clinical features. The impact of APOC1 on PCa was assessed through in vitro experiments. Seven hub genes with AUC values of 0.727-0.926 were identified. APOC1, CFH, NUSAP1 and LGALS1 were highly expressed in bone metastasis tissues, while NR4A2, ADRB2 and ZNF331 exhibited an opposite trend. Immunohistochemistry further confirmed these results. The oxidative phosphorylation pathway was significantly enriched by the identified genes. Aflatoxin B1, benzo(a)pyrene, cyclosporine were identified as potential drugs. APOC1 expression was correlated with clinical features of PCa metastasis. Silencing APOC1 significantly inhibited PCa cell proliferation, clonality, and migration in vitro. This study identified 7 hub genes that potentially facilitate bone metastasis in PCa through mitochondrial metabolic reprogramming. APOC1 emerged as a promising therapeutic target and prognostic marker for PCa with bone metastasis.

bone metastasis cell communication hub genes machine learning prostate cancer single cell analysis

SCI

英语

其他

National Cancer Center Climbing Fund[NCC201925B01] ; Scientific Research Fund of Education Department of Yunnan Province[2022J0246] ; Applied basic research project of yunnan province-kunming medical university joint fund[202101AY070001-160] ; National Natural Science Foundation of China[82160511]

Cell Biology ; Research & Experimental Medicine

Cell Biology ; Medicine, Research & Experimental

WOS:001283152700001

WILEY

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Kunming Med Univ, Peking Univ Canc Hosp Yunnan, Yunnan Canc Hosp, Affiliated Hosp 3,Canc Ctr Yunnan Prov,Dept Urol 1, 519 Kunzhou Rd, Kunming 650199, Yunnan, Peoples R China
2.Kunming Med Univ, Affiliated Hosp 2, Dept Urol 2, 74 Dianmian Blvd, Kunming 650033, Yunnan, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Dept Human Cell Biol & Genet, Shenzhen, Peoples R China
4.Kunming Med Univ, Peking Univ Canc Hosp Yunnan, Yunnan Canc Hosp, Canc Ctr Yunnan Prov,Affiliated Hosp 3,Pathol Dept, Kunming, Yunnan, Peoples R China

Jiang, Haiyang,Liu, Mingcheng,Deng, Yingfei,et al. Identification of prostate cancer bone metastasis related genes and potential therapy targets by bioinformatics and in vitro experiments[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2024,28(15).

Jiang, Haiyang.,Liu, Mingcheng.,Deng, Yingfei.,Zhang, Chongjian.,Dai, Longguo.,...&Yang, Delin.(2024).Identification of prostate cancer bone metastasis related genes and potential therapy targets by bioinformatics and in vitro experiments.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,28(15).

Jiang, Haiyang,et al."Identification of prostate cancer bone metastasis related genes and potential therapy targets by bioinformatics and in vitro experiments".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 28.15(2024).