The Role of SF1 and SF2 Helicases in Biotechnological Applications

Dai, Jing1; Liu, Ronghui2; He, Shujun1; Li, Tie2; Hu, Yuhang2; Huang, Huiqun1; Li, Yi2; Guo, Xinrong1

2024-08-01

10.1007/s12010-024-05027-w

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY   影响因子和分区

0273-2289

1559-0291

Helicases, which utilize ATP hydrolysis to separate nucleic acid duplexes, play crucial roles in DNA and RNA replication, repair, recombination, and transcription. Categorized into the major groups superfamily 1 (SF1) and superfamily 2 (SF2), alongside four minor groups, these proteins exhibit a conserved catalytic core indicative of a shared evolutionary origin while displaying functional diversity through interactions with various substrates. This review summarizes the structures, functions and mechanisms of SF1 and SF2 helicases, with an emphasis on conserved ATPase sites and RecA-like domains essential for their enzymatic and nucleic acid binding capabilities. It highlights the unique 1B and 2B domains in SF1 helicases and their impact on enzymatic activity. The DNA unwinding process is detailed, covering substrate recognition, ATP hydrolysis, and conformational changes, while addressing debates over the active form of UvrD helicase and post-unwinding dissociation. More importantly, this review discusses the biotechnological potential of helicases in emerging technologies such as nanopore sequencing, protein sequencing, and isothermal amplification, focusing on their use in pathogen detection, biosensor enhancement, and cancer treatment. As understanding deepens, innovative applications in genome editing, DNA sequencing, and synthetic biology are anticipated.

SF1 helicase SF2 helicase DNA unwinding Biotechnological application

SCI ; EI

英语

通讯

Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology

Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology

WOS:001283327300003

SPRINGER

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Guangdong Med Univ, Sch Publ Hlth, Dongguan Key Lab Publ Hlth Lab Sci, Dongguan 523808, Peoples R China
2.Southern Univ Sci & Technol, Sch Microelect, Shenzhen 518000, Peoples R China

Dai, Jing,Liu, Ronghui,He, Shujun,et al. The Role of SF1 and SF2 Helicases in Biotechnological Applications[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2024.

Dai, Jing.,Liu, Ronghui.,He, Shujun.,Li, Tie.,Hu, Yuhang.,...&Guo, Xinrong.(2024).The Role of SF1 and SF2 Helicases in Biotechnological Applications.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY.

Dai, Jing,et al."The Role of SF1 and SF2 Helicases in Biotechnological Applications".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2024).