南方科技大学知识苑(SUSTech KC): Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma

题名	Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma
作者	Gao, Xu-Can; Zhou, Biao-Huan; Ji, Zhou-Xin; Li, Qiang ; Liu, Hui-Ning
通讯作者	Gao, Xu-Can
发表日期	2024-07-15
DOI	10.4251/wjgo.v16.i7.3284
发表期刊	WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY 影响因子和分区
ISSN	1948-5204
卷号	16 期号:7
摘要	BACKGROUND Colon adenocarcinoma (COAD) is a malignant tumor of the digestive system. The mechanisms underlying COAD development and progression are still largely unknown. AIM To identify the role of canopy FGF signaling regulator 3 (CNPY3) in the development and progression of COAD by using bioinformatic tools and functional experiments. METHODS Bioinformatic data were downloaded from public databases. The associations of clinicopathological features, survival, and immune function with the expression of CNPY3 were analyzed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses and Gene Set Enrichment Analysis were used to explore the related pathways. Then, quantitative real-time PCR and immunohistochemistry were used for validation of CNPY3 expression in clinical samples and tumor cell lines. Cell lines with CNPY3 knockdown were constructed to further analyze gene functions. The functional experiments included proliferation, invasion, migration and apoptosis assays. RESULTS In both the TCGA cohort and the merged dataset, elevated CNPY3 expression was observed in tumor tissues. High CNPY3 expression correlated with adverse survival and compromised immune functions. Functional enrichment analysis suggested that the pro-oncogenic properties of CNPY3 might be linked to the PI3K-AKT signaling pathway. CNPY3 expression was validated at both the RNA and protein levels. Functional assays indicated that cell proliferation, invasion, and migration were inhibited and cell apoptosis was promoted after CNPY3 knockdown. Additionally, Western blot results revealed the downregulation of key proteins in the PI3K/AKT pathway following CNPY3 knockdown. PI3K/AKT pathway activator reversed the decrease in proliferation, invasion, and migration and the increase in apoptosis. Notably, CNPY3 knockdown still affected the cells when the pathway was inhibited. CONCLUSION This study showed that CNPY3 is upregulated in COAD and might regulate COAD development and progression by the PI3K/AKT pathway. Thus, CNPY3 might be a promising therapeutic target.
关键词	Canopy FGF signaling regulator 3 Colon cancer Biomarker Immune infiltration Prognosis
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
WOS研究方向	Oncology ; Gastroenterology & Hepatology
WOS类目	Oncology ; Gastroenterology & Hepatology
WOS记录号	WOS:001281630600019
出版者	BAISHIDENG PUBLISHING GROUP INC
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/790074
专题	南方科技大学第一附属医院
作者单位	Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Clin Med Coll 2, Affiliated Hosp 1,Dept Anorectal Surg,Jinan Univ, 1017 Dongmen North Rd,Cuizhu St, Shenzhen 518020, Guangdong, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Gao, Xu-Can,Zhou, Biao-Huan,Ji, Zhou-Xin,et al. Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma[J]. WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY,2024,16(7).
APA	Gao, Xu-Can,Zhou, Biao-Huan,Ji, Zhou-Xin,Li, Qiang,&Liu, Hui-Ning.(2024).Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma.WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY,16(7).
MLA	Gao, Xu-Can,et al."Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma".WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY 16.7(2024).