One-Step Physical and Chemical Dual-Reinforcement with Hydrophobic Drug Delivery in Gelatin Hydrogels for Antibacterial Wound Healing

An, Di1; Wang, Zhengkai1; Ning, Yishuo1; Yue, Yuxing1; Xuan, Han1; Hu, Yongjin2; Yang, Mingdi1; Zhou, Haiou1; Liu, Qianqian1; Wang, Xianbiao1; Wang, Ping1; Zhu, Zhiyuan3; Rao, Jingyi2; Zhang, Jingyan1

2024-07-01

10.1021/acsomega.4c01963

ACS OMEGA   影响因子和分区

2470-1343

Gelatin-based bioadhesives, especially methacrylated gelatin (GelMA), have emerged as superior alternatives to sutureless wound closure. Nowadays, their mechanical improvement and therapeutic delivery, particularly for hydrophobic antibiotics, have received ever-increasing interest. Herein, a reinforced gelatin-based hydrogel with a hydrophobic drug delivery property for skin wound treatment was reported. First, photosensitive monomers of N '-(2-nitrobenzyl)-N-acryloyl glycinamide (NBNAGA) were grafted onto GelMA via Michael addition, namely, GelMA-NBNAGA. Second, gelation of the GelMA-NBNAGA solution was accomplished in a few seconds under one step of ultraviolet (UV) light irradiation. Multiple effects were realized simultaneously, including chemical cross-linking initiated by lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), physical cross-linking of uncaged dual hydrogen bonding, and hydrophobic drug release along with o-NB group disintegration. The mechanical properties of the dual-reinforcement hydrogels were verified to be superior to those only with a chemical or physical single-cross-linked network. The hydrophobic anticancer doxorubicin (DOX) and antibiotic rifampicin (Rif) were successfully charged into the hydrogels, separately. The in vitro antimicrobial tests confirmed the antibacterial activity of the hydrogels against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The in vivo wound-healing assessment in mice further assured their drug release and efficacy. Therefore, this NBNAGA-modified GelMA hydrogel has potential as a material in skin wound dressing with a hydrophobic antibiotic on-demand delivery.

SCI

英语

通讯

National Natural Scientific Foundation of China (NNSFC) Project["52003096","21976003","21004001"] ; Opening Foundation of State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology[oic-202301011] ; Anhui Provincial Key Research and Development Project[2022h11020025] ; Natural Science Research Project of Anhui Provincial Education Department["2023AH040037","2022AH020024"] ; Ph.D. Start-up Fund of Anhui Jianzhu University[2020QDZ06]

Chemistry

Chemistry, Multidisciplinary

WOS:001280434600001

AMER CHEMICAL SOC

Web of Science

1.Anhui Jianzhu Univ, Anhui Adv Bldg Mat Int Joint Res Ctr, Sch Mat & Chem Engn, Hefei 230022, Anhui, Peoples R China
2.Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Hubei Engn Res Ctr Biomat & Med Protect Mat, Hubei Key Lab Mat Chem & Serv Failure, Wuhan 430074, Hubei, Peoples R China
3.Southern Univ Sci & Technol, Taizhou Res Inst, Taizhou 318001, Zhejiang, Peoples R China

An, Di,Wang, Zhengkai,Ning, Yishuo,et al. One-Step Physical and Chemical Dual-Reinforcement with Hydrophobic Drug Delivery in Gelatin Hydrogels for Antibacterial Wound Healing[J]. ACS OMEGA,2024.

An, Di.,Wang, Zhengkai.,Ning, Yishuo.,Yue, Yuxing.,Xuan, Han.,...&Zhang, Jingyan.(2024).One-Step Physical and Chemical Dual-Reinforcement with Hydrophobic Drug Delivery in Gelatin Hydrogels for Antibacterial Wound Healing.ACS OMEGA.

An, Di,et al."One-Step Physical and Chemical Dual-Reinforcement with Hydrophobic Drug Delivery in Gelatin Hydrogels for Antibacterial Wound Healing".ACS OMEGA (2024).