Enhancing potential of mesenchymal stem cell exosomes for osteoarthritis by adjusting cell culture condition

Yan，Qian; Qisong，Liu

2025-01-08

10.12307/2024.745

Chinese Journal of Tissue Engineering Research   影响因子和分区

2095-4344

BACKGROUND: Exosomes derived from mesenchymal stem cells are prospective cell-free alternatives for treating osteoarthritis. Their therapeutic activity and clinical transformation potential can be further improved by adjusting cell culture condition. OBJECTIVE: To review the effect of cell culture conditions on exosome activity and to analyze its clinical transformation potential. METHODS: Articles concerning osteoarthritis/cartilage repair and mesenchymal stem cell-derived exosomes were retrieved on CNKI and PubMed using “exosomes, mesenchymal stem cells, osteoarthritis or cartilage repair” as Chinese search terms and “exosomes or extracellular vesicles, mesenchymal stem cells, osteoarthritis or cartilage repair” as English search terms. The search time was from 2010 to 2023. Finally, 100 articles were included for summary and analysis. RESULTS AND CONCLUSION: (1) During cell culture, providing a physical environment favorable for chondrocyte growth or adding chondroprotective small molecules, chondrogenic factors, or inflammatory factors, can further improve the regulatory activity of exosomes secreted by mesenchymal stem cells in maintaining chondrocyte phenotype, promoting cartilage regeneration, and immunosuppression. Therefore, other physical or chemical factors with similar characteristics may also improve the effects of exosomes secreted by mesenchymal stem cells for treating osteoarthritis. (2) Some treatments during cell culture, such as hypoxia induction, pulsed electromagnetic field stimulation, bioreactor, and chondroprotective molecule (e.g., human parathyroid hormone 1-34) stimulation are safe and scalable, which allows for the production of clinical-grade exosomes secreted by mesenchymal stem cells for osteoarthritis treatment. (3) Mesenchymal stem cell exosomes are expected to achieve modified treatment of osteoarthritis, and the feasibility of clinical transformation can be further improved by adjusting the cell culture regimen to enhance the therapeutic activity.

cartilage repair cell culture chondroprotective molecule exosome hypoxia induction inflammatory factor mesenchymal stem cell osteoarthritis pretreatment three-dimensional culture

英语

第一 ; 通讯

2-s2.0-85199669175

Scopus

National Clinical Research Center for Infectious Diseases,Shenzhen Third People’s Hospital,Southern University of Science and Technology,Shenzhen,Guangdong Province,518112,China

Yan，Qian,Qisong，Liu. Enhancing potential of mesenchymal stem cell exosomes for osteoarthritis by adjusting cell culture condition[J]. Chinese Journal of Tissue Engineering Research,2025,29(1):164-174.

Yan，Qian,&Qisong，Liu.(2025).Enhancing potential of mesenchymal stem cell exosomes for osteoarthritis by adjusting cell culture condition.Chinese Journal of Tissue Engineering Research,29(1),164-174.

Yan，Qian,et al."Enhancing potential of mesenchymal stem cell exosomes for osteoarthritis by adjusting cell culture condition".Chinese Journal of Tissue Engineering Research 29.1(2025):164-174.