LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes

Hao，Jianbing; Guo，Xinyu; Wang，Siyu; Guo，Xiaojun; Yuan，Kun; Chen，Ruihong; Hao，Lirong

2024-12-01

10.1038/s41598-024-69323-5

Scientific Reports   影响因子和分区

2045-2322

Urate nephropathy, a common complication of hyperuricemia, has garnered increasing attention worldwide. However, the exact pathogenesis of this condition remains unclear. Currently, inflammation is widely accepted as the key factor in urate nephropathy. Therefore, the aim of this study was to elucidate the interaction of lincRNA-p21/AIF-1/CMPK2/NLRP3 via exosomes in urate nephropathy. This study evaluated the effect of lincRNA-p21/AIF-1/CMPK2/NLRP3 using clinical data collected from patients with urate nephropathy and human renal tubular epithelial cells (HK2) cultured with different concentrations of urate. In clinical research section, the level of lincRNA-p21/AIF-1 in exosomes of urine in patients with hyperuricemia or urate nephropathy was found to be increased, particularly in patients with urate nephropathy. In vitro study section, the level of exosomes, inflammation, autophagy, and apoptosis was increased in HK2 cells induced by urate. Additionally, the expression of lincRNA-p21, AIF-1, CMPK2, and NLRP3 was upregulated in exosomes and HK2 cells. Furthermore, manipulating the activity of lincRNA-p21, AIF-1, CMPK2, and NLRP3 through overexpression or interference vectors regulated the level of inflammation, autophagy, and apoptosis in HK2 cells. In conclusion, the pathway of lincRNA-p21/AIF-1/CMPK2/NLRP3 contributed to inflammation, autophagy, and apoptosis of human renal tubular epithelial cell induced by urate via exosomes. Additionally, the specific exosomes in urine might serve as novel biomarkers for urate nephropathy.

Allograft inflammatory factor 1 Long intergenic noncoding RNA-p21 Mitochondrial nucleotide phosphokinase 2 NLRP3 Urate nephropathy

SCI

英语

第一 ; 通讯

2-s2.0-85200519242

Scopus

Department of Nephrology,Southern University of Science and Technology Hospital,Shenzhen,China

Hao，Jianbing,Guo，Xinyu,Wang，Siyu,et al. LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes[J]. Scientific Reports,2024,14(1).

Hao，Jianbing.,Guo，Xinyu.,Wang，Siyu.,Guo，Xiaojun.,Yuan，Kun.,...&Hao，Lirong.(2024).LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes.Scientific Reports,14(1).

Hao，Jianbing,et al."LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes".Scientific Reports 14.1(2024).