Prolonged premature rupture of membranes with increased risk of infection is associated with gut accumulation of Pseudomonas from the environment

Xiang，Quanhang1; Yan，Xudong2; Shi，Xing1; Huang，Yi'e3; Li，Lingfeng1; Zhong，Jiacheng1; Xu，Tingting1; Tang，Shaohui4; Shi，Wei5; Zhou，Kai1,6

2024-12-01

10.1016/j.csbj.2024.07.007

Computational and Structural Biotechnology Journal   影响因子和分区

2001-0370

Background: Preterm premature rupture of membranes (PPROM) contributes to over one-third of preterm births, and PPROM infants are more susceptible to infections. However, the risk factors remain poorly understood. We here aim to investigate the association of duration of premature rupture of membranes (PROM) and environmental microbiota with the gut microbiota and infection in PPROM infants. Methods: Forty-six premature infants were recruited from two hospitals, and infant fecal and environmental samples were collected. 16 s rRNA sequencing was performed to analyze the fecal and environmental microbiome. Human inflammatory cytokines in cord vein plasma were measured. Results: The gut microbiota composition of PPROM infants was different from that of non-PPROM infants, and the microbiome phenotypes were predicted to be associated with a higher risk of infection, further evidenced by the significantly increased levels of IL-6 and IL-8 in cord vein plasma of PPROM infants. The diversity of the gut microbiota in PPROM infants increased significantly as the duration of PROM excessed 12 h, and Pseudomonas contributed significantly to the dynamic changes. The Pseudomonas species in the gut of PPROM infants were highly homologous to those detected in the ward environment, suggesting that prolonged PROM is associated with horizontal transmission of environmental pathogens, leading to a higher risk of infection. Conclusions: This study highlights that the duration of PROM is associated with the accumulation of environmental pathogens in the gut of PPROM infants, which is a risk factor for nosocomial infections. Improving environmental hygiene could be effective in optimizing the clinical care of PPROM infants.

Environmental microbiota Gut microbiota Premature infants Preterm premature rupture of membranes Pseudomonas

EI

英语

第一

2-s2.0-85197795644

Scopus

1.Shenzhen Institute of Respiratory Diseases,the Second Clinical Medical College (Shenzhen People's Hospital),Jinan University; The First Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,Shenzhen,China
2.Department of Neonatal Intensive Care Unit,the Second Clinical Medical College (Shenzhen People's Hospital),Jinan University,Shenzhen,518020,China
3.Department of Prevention and Healthcare,Shenzhen Baoan Women's and Children's Hospital,Jinan University,Shenzhen,518020,China
4.Department of Gastroenterology,the First Affiliated Hospital,Jinan University,Guangzhou,510632,China
5.Department of Obstetrics,the Second Clinical Medical College (Shenzhen People's Hospital),Jinan University,Shenzhen,518020,China
6.Department of Pathogen Biology,Shenzhen University School of Medicine,Shenzhen,518000,China

Xiang，Quanhang,Yan，Xudong,Shi，Xing,et al. Prolonged premature rupture of membranes with increased risk of infection is associated with gut accumulation of Pseudomonas from the environment[J]. Computational and Structural Biotechnology Journal,2024,23:2851-2860.

Xiang，Quanhang.,Yan，Xudong.,Shi，Xing.,Huang，Yi'e.,Li，Lingfeng.,...&Zhou，Kai.(2024).Prolonged premature rupture of membranes with increased risk of infection is associated with gut accumulation of Pseudomonas from the environment.Computational and Structural Biotechnology Journal,23,2851-2860.

Xiang，Quanhang,et al."Prolonged premature rupture of membranes with increased risk of infection is associated with gut accumulation of Pseudomonas from the environment".Computational and Structural Biotechnology Journal 23(2024):2851-2860.