Zr-MOF Carrier-Enhanced Dual-Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox

Yang, Huiyi1; Zheng, Judun1; Wang, Wei2; Lin, Jingyan3; Wang, Jingru2; Liu, Lunjing4; Wu, Wenjie2; Zhang, Chengli1; Zhang, Mingxia3; Fu, Yu4; Yang, Bin1; Liao, Yuhui2,4

2024-08-01

10.1002/advs.202405848

ADVANCED SCIENCE   影响因子和分区

2198-3844

["Dual-mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6-Ru/Pt NPs is constructed for dual-mode platforms by integrating double-layered ECL luminophores and the nanozyme using Zr-MOF (UiO-66-NH2) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual-mode platforms are used within 15 min from the \"sample in\" to the \"result out\" steps, without nucleic acid amplification. The colorimetric mode allows the screening of MPXV with the visual limit of detection (vLOD) of 0.1 pM (6 x 108 copies mu L-1) and the ECL mode supports quantitative detection of MPXV with an LOD as low as 10 aM (6 copiesmu L-1), resulting in a broad sensing range of 60 to 3 x 1011 copiesmu L-1 (10 orders of magnitude). Validation is conducted using 50 clinical samples, which is 100% concordant to those of quantitative polymerase chain reaction (qPCR), indicating that Ru@U6-Ru/Pt NPs-based dual-mode sensing platforms showed great promise as rapid, sensitive, and accurate tools for diagnosis of the nucleic acid of MPXV and other infectious pathogens.","A new nanotag, Ru@U6-Ru/Pt NPs is constructed for dual-mode platforms by integrating double-layered electrochemiluminescence luminophores and the nanozyme using Zr-MOF as the carrier. This dual-mode sensing platform shows great promise as a rapid, sensitive, and accurate tool for point-of-care testing and clinical diagnosis of nucleic acids of monkeypox virus and other infectious pathogens. image"]

amplification-free colorimetric-ECL dual-mode monkeypox virus Ru@U6-Ru/Pt NPs nanotag signal amplification

SCI ; EI

英语

通讯

National Key R&D Program of China["2021YFC2302200","2023YFC2307402"] ; National Natural Science Foundation of China["82322042","82272248"] ; Natural Science Fund of Guangdong Province for Distinguished Young Scholars[2022B1515020089] ; Zhongnanshan Medical Foundation of Guangdong Province[ZNSA-2021012] ; China Postdoctoral Science Foundation[2024M751305] ; Guangdong Province Science and Technology Plan Project "Biosafety Technology" Special Project, Development of High-Level Life Safety Experimental Air Intelligent Machine installation System[2022B111010003] ; Guangdong Basic and Applied Basic Research Foundation[2023A1515140123]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001286622800001

WILEY

Web of Science

1.Southern Med Univ, Dermatol Hosp, Mol Diag & Treatment Ctr Infect Dis, Guangzhou 510000, Peoples R China
2.Kunming Med Univ, Inst Engn Med, Kunming 650500, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen 518000, Peoples R China
4.Ningxia Med Univ, NHC Key Lab Metab Cardiovasc Dis Res, Ningxia Key Lab Vasc Injury & Repair Res, Yinchuan 750004, Peoples R China

Yang, Huiyi,Zheng, Judun,Wang, Wei,et al. Zr-MOF Carrier-Enhanced Dual-Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox[J]. ADVANCED SCIENCE,2024.

Yang, Huiyi.,Zheng, Judun.,Wang, Wei.,Lin, Jingyan.,Wang, Jingru.,...&Liao, Yuhui.(2024).Zr-MOF Carrier-Enhanced Dual-Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox.ADVANCED SCIENCE.

Yang, Huiyi,et al."Zr-MOF Carrier-Enhanced Dual-Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox".ADVANCED SCIENCE (2024).