南方科技大学知识苑(SUSTech KC): NOR1 promotes the osteoblastic differentiation of human periodontal ligament stem cells via TGF-Î² signaling pathway

题名	NOR1 promotes the osteoblastic differentiation of human periodontal ligament stem cells via TGF-Î² signaling pathway
作者	Wu, Yun 1,2,3,4; Jing, Huan 1,2,3,4; Li, Yicun 1,2,3,4; Li, Mengqing 5; Zheng, Yating 5; Lin, Yuntao 1,2,3,4; Ma, Guixing 6 ; Cao, Huiling6 ; Yang, Hongyu 1,2,3,4
通讯作者	Cao, Huiling; Yang, Hongyu
发表日期	2024-12-01
DOI	10.1007/s00018-024-05356-3
发表期刊	CELLULAR AND MOLECULAR LIFE SCIENCES 影响因子和分区
ISSN	1420-682X
EISSN	1420-9071
卷号	81 期号:1
摘要	Alveolar bone loss is a main manifestation of periodontitis. Human periodontal ligament stem cells (PDLSCs) are considered as optimal seed cells for alveolar bone regeneration due to its mesenchymal stem cell like properties. Osteogenic potential is the premise for PDLSCs to repair alveolar bone loss. However, the mechanism regulating osteogenic differentiation of PDLSCs remain elusive. In this study, we identified Neuron-derived orphan receptor 1 (NOR1), was particularly expressed in PDL tissue in vivo and gradually increased during osteogenic differentiation of PDLSCs in vitro. Knockdown of NOR1 in hPDLSCs inhibited their osteogenic potential while NOR1 overexpression reversed this effect. In order to elucidate the downstream regulatory network of NOR1, RNA-sequencing was used. We found that downregulated genes were mainly enriched in TGF-beta, Hippo, Wnt signaling pathway. Further, by western blot analysis, we verified that the expression level of phosphorylated-SMAD2/3 and phosphorylated-SMAD4 were all decreased after NOR1 knockdown. Additionally, ChIP-qPCR and dual luciferase reporter assay indicated that NOR1 could bind to the promoter of TGFBR1 and regulate its activity. Moreover, overexpression of TGFBR1 in PDLSCs could rescue the damaged osteogenic potential after NOR1 knockdown. Taken together, our results demonstrated that NOR1 could activate TGF-beta/SMAD signaling pathway and positively regulates the commitment of osteoblast lineages of PDLSCs by targeting TGFBR1 directly.
关键词	Periodontal ligament stem cell (PDLSC) Osteogenic differentiation Neuron-derived orphan receptor 1 (NOR1) Transforming growth factor beta receptor 1 (TGFBR1) TGF-beta signaling pathway
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:001287692600002
出版者	SPRINGER BASEL AG
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/803301
专题	南方科技大学医学院_生物化学系南方科技大学医学院
作者单位	1.Peking Univ, Shenzhen Peking Univ Hong Kong Univ Sci & Technol, Shenzhen Hosp, Inst Stomatol, Shenzhen 518000, Guangdong, Peoples R China 2.Guangdong Prov High Level Clin Key Specialty, Shenzhen 518000, Guangdong, Peoples R China 3.Guangdong Prov Engn Res Ctr Oral Dis Diag & Treatm, Shenzhen 518000, Guangdong, Peoples R China 4.Peking Univ, Shenzhen Hosp, Stomatol Ctr, Dept Oral & Maxillofacial Surg, Shenzhen 518000, Guangdong, Peoples R China 5.Peking Univ, Shenzhen Hosp, Dept Pathol, Shenzhen 518000, Guangdong, Peoples R China 6.Southern Univ Sci & Technol, Key Univ Lab Metab & Hlth Guangdong, Sch Med, Dept Biochem,Guangdong Prov Key Lab Cell Microenvi, Shenzhen 518055, Peoples R China
通讯作者单位	生物化学系; 南方科技大学医学院
推荐引用方式 GB/T 7714	Wu, Yun,Jing, Huan,Li, Yicun,et al. NOR1 promotes the osteoblastic differentiation of human periodontal ligament stem cells via TGF-Î² signaling pathway[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2024,81(1).
APA	Wu, Yun.,Jing, Huan.,Li, Yicun.,Li, Mengqing.,Zheng, Yating.,...&Yang, Hongyu.(2024).NOR1 promotes the osteoblastic differentiation of human periodontal ligament stem cells via TGF-Î² signaling pathway.CELLULAR AND MOLECULAR LIFE SCIENCES,81(1).
MLA	Wu, Yun,et al."NOR1 promotes the osteoblastic differentiation of human periodontal ligament stem cells via TGF-Î² signaling pathway".CELLULAR AND MOLECULAR LIFE SCIENCES 81.1(2024).