Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis

Pan, Yajie1,2,3; Yu, Bofan1,3; Wei, Xin1,3; Qiu, Yuping3; Mao, Xin3; Liu, Yuelin3; Yan, Wei3; Linghu, Qianyan3; Li, Wenyang3; Guo, Hongwei3; Tang, Zhonghua1,2

2024-08-01

10.1093/plcell/koae224

PLANT CELL   影响因子和分区

1040-4651

1532-298X

["Identifying the essential factors and underlying mechanisms regulating plant heat stress (HS) responses is crucial for mitigating the threat posed by HS on plant growth, development, distribution, and productivity. In this study, we found that the Arabidopsis (Arabidopsis thaliana) super-killer2 (ski2) dicer-like4 (dcl4) mutant, characterized by RNA processing defects and the accumulation of abundant 22-nt small interfering RNAs derived from protein-coding transcripts, displayed significantly increased expression levels of HS-responsive genes and enhanced thermotolerance. These traits primarily resulted from the suppression of SMAX1-LIKE4 (SMXL4) and SMXL5, which encode 2 putative transcriptional regulators that belong to the SMXL protein family. While smxl4 and smxl5 single mutants were similar to wild type, the smxl4 smxl5 double mutant displayed substantially heightened seedling thermotolerance. Further investigation demonstrated that SMXL4 and SMXL5 repressed the transcription of HEAT-SHOCK TRANSCRIPTION FACTOR A2 (HSFA2), encoding a master regulator of thermotolerance, independently of ethylene-response factor-associated amphiphilic repression motifs. Moreover, SMXL4 and SMXL5 interacted with HSFA1d and HSFA1e, central regulators sensing and transducing HS stimuli, and antagonistically affected their transactivation activity. In addition, HSFA2 directly bound to the SMXL4 and SMXL5 promoters, inducing their expression during recovery from HS. Collectively, our findings elucidate the role of the SMXL4/SMXL5-HSFA2 regulatory module in orchestrating plant thermotolerance under HS.","The elevated temperatures inhibit 2 suppressor proteins, leading to the activation of 2 crucial regulators that detect and relay heat stress (HS) signals, ultimately triggering plant responses to HS."]

SCI

英语

通讯

Shenzhen Science and Technology Innovation Commission Program[20200925161843002] ; National Natural Science Foundation of China["32261160572","31870254"] ; National Key Research and Development Program of China[2022YFF1300500] ; Shenzhen Science and Technology Program["JCYJ20170817105503416","JCYJ20190809163019421"] ; New Cornerstone Science Foundation[NCl202235]

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

WOS:001293071600001

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Harbin 150040, Peoples R China
2.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
3.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Sch Life Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China

Pan, Yajie,Yu, Bofan,Wei, Xin,et al. Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis[J]. PLANT CELL,2024.

Pan, Yajie.,Yu, Bofan.,Wei, Xin.,Qiu, Yuping.,Mao, Xin.,...&Tang, Zhonghua.(2024).Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis.PLANT CELL.

Pan, Yajie,et al."Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis".PLANT CELL (2024).