Abundant mRNA m¹A modification in dinoflagellates: a new layer of gene regulation

Li, Chongping1,2,3; Li, Ying1,2,3,4,5; Guo, Jia2,3,6; Wang, Yuci1,2,3,5; Shi, Xiaoyan1,2,3; Zhang, Yangyi1,2,3; Liang, Nan1,2,3; Ma, Honghui7; Yuan, Jie4; Xu, Jiawei2,3,6; Chen, Hao1,2,3,5,6

2024-09-01

10.1038/s44319-024-00234-2

EMBO REPORTS   影响因子和分区

1469-221X

1469-3178

Dinoflagellates, a class of unicellular eukaryotic phytoplankton, exhibit minimal transcriptional regulation, representing a unique model for exploring gene expression. The biosynthesis, distribution, regulation, and function of mRNA N1-methyladenosine (m(1)A) remain controversial due to its limited presence in typical eukaryotic mRNA. This study provides a comprehensive map of m(1)A in dinoflagellate mRNA and shows that m1A, rather than N6-methyladenosine (m6A), is the most prevalent internal mRNA modification in various dinoflagellate species, with an asymmetric distribution along mature transcripts. In Amphidinium carterae, we identify 6549 m(1)A sites characterized by a non-tRNA T-loop-like sequence motif within the transcripts of 3196 genes, many of which are involved in regulating carbon and nitrogen metabolism. Enriched within 3 ' UTRs, dinoflagellate mRNA m(1)A levels negatively correlate with translation efficiency. Nitrogen depletion further decreases mRNA m(1)A levels. Our data suggest that distinctive patterns of m(1)A modification might influence the expression of metabolism-related genes through translational control.

N1-methyladenosine Dinoflagellates Post-transcriptional Regulation mRNA Translation

SCI

英语

第一 ; 通讯

Center for Computational Science and Engineering at Southern University of Science and Technology["2022YFC2702705","2019YFA0110900","2019YFA0802200"] ; National Key Research and Development Program of China["32170819","32170604"] ; National Natural Science Foundation of China[2021QN02Y122] ; Pearl River Recruitment Program of Talents[B2021032] ; Health Department of Guangdong Province[ZDSYS20200811144002008] ; Shenzhen Key Laboratory of Gene Regulation and Systems Biology[20231120115406001] ; Shenzhen Innovation Committee of Science and Technology and Funding for Scientific Research[24IRTSTHN037] ; Scientific and Technological Innovation Team Project of Universities[222301420013] ; Young and Middle-aged Academic Leaders and the Leading Talents of Henan Health Commission[ZYCXTD2023004]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:001303890100001

SPRINGERNATURE

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Southern Univ Sci & Technol, Sch Med, Joint Lab Guangdong & Hongkong Univ Vasc Homeostas, Dept Human Cell Biol & Genet, Shenzhen 518000, Peoples R China
2.Zhengzhou Univ, First Affiliated Hosp, Zhengzhou 450000, Peoples R China
3.Henan Acad Innovat Med Sci, Inst Reprod Hlth, Zhengzhou 450000, Peoples R China
4.Shenzhen Peoples Hosp, 3046 Shennan E Rd, Shenzhen 518020, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Key Lab Gene Regulat & Syst Biol, Shenzhen 518000, Peoples R China
6.NHC Key Lab Birth Defects Prevent, Zhengzhou 450000, Peoples R China
7.Fudan Univ, Dept Liver Surg & Transplantat,Inst Biomed Sci, Liver Canc Inst,Minist Educ,Key Lab Med Epigenet &, Zhongshan Hosp,Key Lab Carcinogenesis & Canc Invas, Shanghai 200000, Peoples R China

Li, Chongping,Li, Ying,Guo, Jia,et al. Abundant mRNA m¹A modification in dinoflagellates: a new layer of gene regulation[J]. EMBO REPORTS,2024.

Li, Chongping.,Li, Ying.,Guo, Jia.,Wang, Yuci.,Shi, Xiaoyan.,...&Chen, Hao.(2024).Abundant mRNA m¹A modification in dinoflagellates: a new layer of gene regulation.EMBO REPORTS.

Li, Chongping,et al."Abundant mRNA m¹A modification in dinoflagellates: a new layer of gene regulation".EMBO REPORTS (2024).