Genome-wide characterization of extrachromosomal circular DNA in SLE and functional analysis reveal their association with apoptosis

Li, Yixi1,2; Ge, Fangfang3; Liu, Chengxun3; Pu, Wenjun2; Lv, Wei3; Zeng, Zhipeng2; Yin, Lianghong4; Liu, Dongzhou5; Li, Yasong1; Tang, Donge2; Han, Peng3; Dai, Yong6,7

2024-11-01

10.1016/j.trsl.2024.08.004

TRANSLATIONAL RESEARCH   影响因子和分区

1931-5244

1878-1810

Extrachromosomal circular DNA (eccDNA) derived from linear chromosomes, are showed typical nucleosomal ladder pattern in agarose gel which as a known feature of apoptosis and demonstrated to be immunogenicity. In systemic lupus erythematosus (SLE) patients, elevated levels of cell-free DNA (cfDNA) can be found in either linear forms or circular forms, while circular ones are much less common and harder to detect. The molecular characteristics and function of circular forms in plasma SLE patients remains elusive. Herein, we characterized the hallmarks of plasma eccDNA in SLE patients, including the lower normalized number and GC content of eccDNA in SLE plasma than in the healthy, and SLE eccDNA number positively correlated with C3 and negatively with anti-dsDNA antibodies. The differential eccGenes (eccDNAs carrying the protein coding gene sequence) of SLE was significantly enriched in apoptosis-related pathways. The artificially synthesized eccDNA with sequences of the PRF1 exon region could promote transcriptional expression of PRF1, IFNA and IFIT3 and inhibit early-stage apoptosis. Plasma eccDNA can serve as a novel autoantigen in the pathogenesis of SLE.

eccDNA Immunogenicity Apoptosis Lupus nephritis

SCI

英语

通讯

Science and technology plan of Shenzhen[JCYJ20210324113214039] ; Guangxi Key laboratory of Metabolic Diseases Research[20-065-76] ; Natural Science Foundation of Guangxi Zhuang Autonomous Region[2017GXNSFAA198375]

Medical Laboratory Technology ; General & Internal Medicine ; Research & Experimental Medicine

Medical Laboratory Technology ; Medicine, General & Internal ; Medicine, Research & Experimental

WOS:001303100700001

ELSEVIER SCIENCE INC

Web of Science

1.Zhejiang Prov Peoples Hosp, Affiliated Peoples Hosp, Hangzhou Med Coll, Ctr Gen Practice Med,Dept Rheumatol & Immunol, Hangzhou 310000, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Guangdong Prov Autoimmune Dis Precis Med Engn Res, Clin Med Coll 2,Shenzhen Autoimmune Dis Engn Res C, Shenzhen 518020, Peoples R China
3.Qingdao Europe Adv Inst Life Sci, Lars Bolund Inst Regenerat Med, BGI Res, Qingdao 266555, Peoples R China
4.Jinan Univ, Affiliated Hosp 1, Inst Nephrol & Blood Purificat, Guangzhou 510630, Peoples R China
5.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Rheumatol & Immunol, Shenzhen 518020, Peoples R China
6.Anhui Univ Sci & Technol, Affiliated Hosp 1, Sch Med, Huainan 232001, Peoples R China
7.Southern Univ Sci & Technol, Taizhou Res Inst, Comprehens Hlth Ind Res Ctr, Taizhou 318000, Peoples R China

Li, Yixi,Ge, Fangfang,Liu, Chengxun,et al. Genome-wide characterization of extrachromosomal circular DNA in SLE and functional analysis reveal their association with apoptosis[J]. TRANSLATIONAL RESEARCH,2024,273.

Li, Yixi.,Ge, Fangfang.,Liu, Chengxun.,Pu, Wenjun.,Lv, Wei.,...&Dai, Yong.(2024).Genome-wide characterization of extrachromosomal circular DNA in SLE and functional analysis reveal their association with apoptosis.TRANSLATIONAL RESEARCH,273.

Li, Yixi,et al."Genome-wide characterization of extrachromosomal circular DNA in SLE and functional analysis reveal their association with apoptosis".TRANSLATIONAL RESEARCH 273(2024).