A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells

Liang, Fuchao; Ke, Mi; Tian, Ruijun

2024-07-01

10.3724/SP.J.1123.2024.01016

CHINESE JOURNAL OF CHROMATOGRAPHY   影响因子和分区

1000-8713

Tyrosine phosphorylation, a common post-translational modification process for proteins, is involved in a variety of biological processes. However, the abundance of tyrosine-phosphorylated proteins is very low, making their identification by mass spectrometry (MS) is difficult; thus, milligrams of the starting material are often required for their enrichment. For example, tyrosine phosphorylation plays an important role in T cell signal transduction. However, the number of primary T cells derived from biological tissue samples is very small, and these cells are difficult to culture and expand; thus, the study of T cell signal transduction is usually carried out on immortalized cell lines, which can be greatly expanded. However, the data from immortalized cell lines cannot fully mimic the signal transduction processes observed in the real physiological state, and they usually lead to conclusions that are quite different from those of primary T cells. Therefore, a highly sensitive proteomic method was developed for studying tyrosine phosphorylation modification signals in primary T cells. To address the issue of the limited T cells numbers, a comprehensive protocol was first optimized for the isolation, activation, and expansion of primary T cells from mouse spleen. CD3+ primary T cells were successfully sorted; more than 91% of the T cells collected were well activated on day 2, and the number of T cells expanded to over 7-fold on day 4. Next, to address the low abundance of tyrosine-phosphorylated proteins, we used SH2-superbinder affinity enrichment and immobilized Ti(4+)affinity chromatography (Ti4+-IMAC) to enrich the tyrosine-phosphorylated polypeptides of primary T cells that were co-stimulated with anti-CD3 and anti-CD28. These polypeptides were resolved using nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS). Finally, 282 tyrosine phosphorylation sites were successfully identified in 1 mg of protein, including many tyrosine phosphorylation sites on the immunoreceptor tyrosine-based activation motif (ITAM) in the intracellular region of the T cell receptor membrane protein CD3, as well as the phosphotyrosine sites of ZAP70, LAT, VAV1, and other proteins related to signal transduction under costimulatory conditions. In summary, to solve the technical problems of the limited number of primary cells, low abundance of tyrosine-phosphorylated proteins, and difficulty of detection by MS, we developed a comprehensive proteomic method for the in-depth analysis of tyrosine phosphorylation modification signals in primary T cells. This protocol may be applied to map signal transduction networks that are closely related to physiological states.

nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) tyrosine phosphorylation primary T cell co-stimulation signal transduction

ESCI

中文

第一 ; 通讯

National Key Research and Development Program of China["2021YFA1301601","2021YFA1301602","2021YFA1302603"] ; National Natural Science Foundation of China["92253304","22125403"] ; Shenzhen Innovation of Science and Technology Commission["JSGGZD20220822095200001","JCYJ20210324120210029"]

Chemistry

Chemistry, Analytical

WOS:001301255900009

SCIENCE PRESS

Web of Science

Southern Univ Sci & Technol, Coll Sci, Dept Chem, Shenzhen 518055, Peoples R China

Liang, Fuchao,Ke, Mi,Tian, Ruijun. A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells[J]. CHINESE JOURNAL OF CHROMATOGRAPHY,2024,42(7).

Liang, Fuchao,Ke, Mi,&Tian, Ruijun.(2024).A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells.CHINESE JOURNAL OF CHROMATOGRAPHY,42(7).

Liang, Fuchao,et al."A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells".CHINESE JOURNAL OF CHROMATOGRAPHY 42.7(2024).