Light-Switch Electrochemiluminescence-Driven microfluidic sensor for rapid and sensitive detection of Mpox virus

Fu, Yu1; Chen, Xu2; Song, Wenlu4; Kuang, Jiahua5; Wu, Wenjie1; Yang, Xiongtiao1; Xia, Jiaojiao1; Liu, Lunjing3; Yang, Yang5; Ma, Shengchao3; Jiang, Yideng3; Liao, Yuhui2,3,4

2024-10-15

10.1016/j.cej.2024.154930

CHEMICAL ENGINEERING JOURNAL   影响因子和分区

1385-8947

1873-3212

Rapid, real-time, and accurate detection of pathogenic nucleic acids is crucial for the control and treatment of Mpox virus infection; however, classical detection methods are limited by time-consuming amplification steps, cumbersome equipment, and the requirement for professional operators. Herein, we developed an electrochemiluminescence (ECL)-driven microfluidic detection chip based on a light-switch molecule, which when coupled with rapid recombinase polymerase amplification (RPA) enabled the detection of Mpox within 30 min. [Ru(phen)2dppz]2+ was used as the ECL light-switch molecule owing to its significant ECL signal enhancement property when intercalated into double-stranded RPA products. The microfluidic chip included two independent liquid storage tanks preloaded with an RPA reagent mixture and [Ru(phen)2dppz]2+, which were mixed via centrifugation to produce ECL signals. The developed sensing platform effectively detected Mpox virus with high sensitivity, and the limit of detection was as low as 10 copies/mu L. Moreover, it showed 100 % sensitivity and 100 % specificity when validated against the quantitative polymerase chain reaction assay using clinical samples from the pustules, throat, urine, blood, saliva, and anus of Mpox-positive patients. Combined with a hand-held detection device, this platform can facilitate the early diagnosis of Mpox cases in point-of-care testing without trained personnel and specialized equipment.

Mpox Recombinase polymerase amplification Electrochemiluminescence Nucleic acid detection Point-of-care testing

SCI ; EI

英语

通讯

National Key R & D Program of China["2021YFC2302200","2023YFC2307402"] ; National Natural Science Foundation of China["82322042","82272248","82002244"] ; Natural Science Fund of Guangdong Province for Distinguished Young Scholars[2022B1515020089] ; Guangdong Science and Technology Plan Project, Construction of high-level biosafety laboratories[2021B1212030010] ; Zhongnanshan Medical Foundation of Guangdong Province[ZNSA-2021012]

Engineering

Engineering, Environmental ; Engineering, Chemical

WOS:001302263900001

ELSEVIER SCIENCE SA

ENGINEERING

Web of Science

1.Longgang Dist Cent Hosp Shenzhen, Shenzhen 518000, Peoples R China
2.Kunming Med Univ, Inst Engn Med, Kunming 650500, Peoples R China
3.Ningxia Med Univ, NHC Key Lab Metab Cardiovasc Dis Res, Ningxia Key Lab Vasc Injury & Repair Res, Yinchuan 750004, Peoples R China
4.Southern Med Univ, Dermatol Hosp, Mol Diag & Treatment Ctr Infect Dis, Guangzhou 510000, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Hosp Affiliated 2,Shenzhen Key Lab Pathogen & Immu, Natl Clin Res Ctr Infect Dis,State Key Discipline, Shenzhen 518000, Peoples R China

Fu, Yu,Chen, Xu,Song, Wenlu,et al. Light-Switch Electrochemiluminescence-Driven microfluidic sensor for rapid and sensitive detection of Mpox virus[J]. CHEMICAL ENGINEERING JOURNAL,2024,498.

Fu, Yu.,Chen, Xu.,Song, Wenlu.,Kuang, Jiahua.,Wu, Wenjie.,...&Liao, Yuhui.(2024).Light-Switch Electrochemiluminescence-Driven microfluidic sensor for rapid and sensitive detection of Mpox virus.CHEMICAL ENGINEERING JOURNAL,498.

Fu, Yu,et al."Light-Switch Electrochemiluminescence-Driven microfluidic sensor for rapid and sensitive detection of Mpox virus".CHEMICAL ENGINEERING JOURNAL 498(2024).