南方科技大学知识苑(SUSTech KC): Dynamic monitoring of SO2 changes during ferroptosis using a light-controlled lipid droplets-targeting probe

题名	Dynamic monitoring of SO2 changes during ferroptosis using a light-controlled lipid droplets-targeting probe
作者	Wang, Ziqiang1 ; Xiao, Ziyu2 ; Pan, Tingting 3; Liao, Jianxiang 3; Tian, Yanqing2
通讯作者	Pan, Tingting; Liao, Jianxiang; Tian, Yanqing
发表日期	2024-12-01
DOI	10.1016/j.snb.2024.136470
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL 影响因子和分区
EISSN	0925-4005
卷号	420
摘要	During the process of ferroptosis, the generation of toxic lipid peroxides triggers severe inflammatory responses. Despite sulfur dioxide (SO2) being recognized as an important signal molecule and antioxidant, its specific physiological mechanism in ferroptosis remains unclear. Here, we developed a novel probe, SP-TB, tailored for detecting SO2 within lipid droplets (LDs). SP-TB features a triphenylamine moiety for LD targeting, coupled with a spiropyran serving as both the SO2 reaction site and a light-responsive switch. Under normal conditions, SP-TB exhibits yellow fluorescence at 570 nm with its SO2 reaction site closed. Upon UV light activation, the spiropyran moiety undergoes isomerization to the merocyanine form (MC-TB), resulting in red fluorescence emission at 634 nm. During this photoisomerization, the exposed C--C-C--N+ fragment enables specific Michael addition reactions with SO2, quenching the red fluorescence of MC-TB and enhancing yellow fluorescence. The probe exhibits stable photoreversibility and excellent LDs targeting, along with remarkable SO2 selectivity with a low detection limit of 3.5 mu M. Notably, the controllability of the SO2 reaction site minimizes false positives. This probe was successfully used to monitor the changes of SO2 concentrations in LDs during ferroptosis.
关键词	Ferroptosis Sulfur dioxide Lipid droplets Spiropyran Fluorescent probe
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	第一 ; 通讯
资助项目	Shenzhen Key Medical Discipline Construction Fund[SZXK033] ; Shenzhen Fund for Guangdong Provincial High level Clinical Key Specialties[SZGSP012] ; Guangdong Basic and Applied Basic Research Foundation[2023A1515110085] ; SUSTech[Y01256114] ; Guangdong-Hong Kong-Macau Joint Laboratory on Micro-Nano Manufacturing Technology[2021LSYS004]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:001301336200001
出版者	ELSEVIER SCIENCE SA
ESI学科分类	CHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/805131
专题	南方科技大学医学院工学院_材料科学与工程系
作者单位	1.Southern Univ Sci & Technol, Sch Med, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Dept Mat Sci & Engn, 1088 Xueyuan Blvd, Shenzhen 518055, Guangdong, Peoples R China 3.Shenzhen Childrens Hosp, Dept Pediat Neurol, 7019 Yitian Rd, Shenzhen 518038, Peoples R China
第一作者单位	南方科技大学医学院
通讯作者单位	材料科学与工程系
第一作者的第一单位	南方科技大学医学院
推荐引用方式 GB/T 7714	Wang, Ziqiang,Xiao, Ziyu,Pan, Tingting,et al. Dynamic monitoring of SO2 changes during ferroptosis using a light-controlled lipid droplets-targeting probe[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,420.
APA	Wang, Ziqiang,Xiao, Ziyu,Pan, Tingting,Liao, Jianxiang,&Tian, Yanqing.(2024).Dynamic monitoring of SO2 changes during ferroptosis using a light-controlled lipid droplets-targeting probe.SENSORS AND ACTUATORS B-CHEMICAL,420.
MLA	Wang, Ziqiang,et al."Dynamic monitoring of SO2 changes during ferroptosis using a light-controlled lipid droplets-targeting probe".SENSORS AND ACTUATORS B-CHEMICAL 420(2024).