Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers

Wang，Keyun1,2; Zhang，Li3,4; Zhang，Sirui5; Liu，Ye6; Mao，Jiawei1,2; Liu，Zhen1,2; Xu，Lin3; Li，Kejia1,2; Wang，Jianshu3; Ma，Yanni1,2; Wang，Jiayi1,2; Li，Haitao7; Wang，Zefeng5,8; Li，Guohui6; Cheng，Hong3,4; Ye，Mingliang1,2

2024-12-01

10.1038/s41467-024-51979-2

Nature Communications   影响因子和分区

2041-1723

Protein methylation is a functionally important post-translational modification that occurs on diverse amino acid residues. The current proteomics approaches are inefficient to discover the methylation on residues other than Arg and Lys, which hinders the deep understanding of the functional role of rare protein methylation. Herein, we present a methyl-specific metabolic labeling approach for global methylome mapping, which enable the acquisition of methylome dataset covering diverse methylation types. Interestingly, of the identified methylation events, His methylation is found to be preferably occurred in C3H1 zinc fingers (ZFs). These His methylation events are determined to be Nπ specific and catalyzed by CARNMT1. The His methylation is found to stabilize the structure of ZFs. U2AF1 is used as a proof-of-concept to highlight the functional importance of His methylation in ZFs in RNA binding and RNA metabolism. The results of this study enable novel understanding of how protein methylation regulates cellular processes.

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其他

2-s2.0-85202739470

Scopus

1.State Key Laboratory of Medical Proteomics,CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
2.University of Chinese Academy of Sciences,Beijing,100049,China
3.Key Laboratory of RNA Innovation,Science and Engineering,Shanghai Key Laboratory of Molecular Andrology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,200031,China
4.Key Laboratory of Systems Health Science of Zhejiang Province,School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,310024,China
5.CAS Key Laboratory of Computational Biology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,200031,China
6.Laboratory of Molecular Modeling and Design,State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
7.MOE Key Laboratory of Protein Sciences,Beijing Frontier Research Center for Biological Structure,School of Medicine,Tsinghua University,Beijing,100084,China
8.School of Life Science,Southern University of Science and Technology,Shenzhen,China

Wang，Keyun,Zhang，Li,Zhang，Sirui,et al. Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers[J]. Nature Communications,2024,15(1).

Wang，Keyun.,Zhang，Li.,Zhang，Sirui.,Liu，Ye.,Mao，Jiawei.,...&Ye，Mingliang.(2024).Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers.Nature Communications,15(1).

Wang，Keyun,et al."Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers".Nature Communications 15.1(2024).