南方科技大学知识苑(SUSTech KC): Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice

题名	Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice
作者	Yan, Bin 1; Liu, Chunyan 1; Sun, Jing 1,2; Mao, Yang 3; Zhou, Can 1,4; Li, Ji 1,4; Liu, Wei 1; Li, Shengdong 1,4; Yan, Wei 5 ; Fu, Chenjian 6; Qin, Peng 6; Fu, Xingxue 6; Zhao, Xinghui 6; Song, Xianwei 7; Nie, Jiawei 3,4; Gao, Feng 3; Yang, Yuanzhu 6,8,9; Chen, Yuhang 3,4; Cao, Xiaofeng 1,4
通讯作者	Liu, Chunyan; Yang, Yuanzhu; Chen, Yuhang; Cao, Xiaofeng
发表日期	2024-09-01
DOI	10.1038/s41422-024-01012-4
发表期刊	CELL RESEARCH 影响因子和分区
ISSN	1001-0602
EISSN	1748-7838
摘要	Hybrid rice, widely planted in Asia, is pathogen resistant and has superior yields, making it a major contributor to global food security. The two-line hybrid rice system, which utilizes mutants exhibiting photo-/thermo-sensitive genic male sterility (P/TGMS), is the leading hybrid rice breeding technology. Mutations in THERMO-SENSITIVE GENIC MALE STERILE 5 (TMS5) accounts for over 95% of current TGMS lines. We previously found that tms5 carries a mutation in ribonuclease ZS1. Despite its importance for breeding robust rice lines, the mechanism underlying tms5-mediated TGMS remains elusive. Here, we demonstrate that TMS5 is a tRNA 2 ',3 '-cyclic phosphatase. The tms5 mutation leads to accumulation of 2 ',3 '-cyclic phosphate (cP)-Delta CCA-tRNAs (tRNAs without 3 ' CCA ended with cP), which is exacerbated by high temperatures, and reduction in the abundance of mature tRNAs, particularly alanine tRNAs (tRNA-Alas). Overexpression of tRNA-Alas in the tms5 mutant restores male fertility to 70%. Remarkably, male fertility of tms5 mutant is completely restored at high temperatures by knocking out OsVms1 which encodes the enzyme for cP-Delta CCA-tRNA generation. Our study reveals the mechanism underlying tms5-mediated TGMS in rice and provides mechanistic insight into the further improvement of TGMS in hybrid crop development.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Ministry of Agriculture and Rural Affairs of the People's Republic of China (MOA)[2023ZD04073] ; Biological Breeding-National Science and Technology Major Project of China[2020YFA0509900] ; National Key R&D Program of China["32330019","32171284","32170620","32370633"] ; National Natural Science Foundation of China[XDA24010302]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:001308255000001
出版者	SPRINGERNATURE
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/828643
专题	南方科技大学-北京大学植物与食品联合研究所生命科学学院_生物系
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 2.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Agr Genet Resources & Biotechnol, Inst Biotechnol, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Lab Mol Dev Biol, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen, Guangdong, Peoples R China 6.Yuan Longping High Tech Agr Co Ltd, Changsha, Hunan, Peoples R China 7.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Seed Innovat, Beijing, Peoples R China 8.State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China 9.Minist Agr & Rural Affairs, Key Lab Rice Germplasm Enhancement Southern China, Changsha, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Yan, Bin,Liu, Chunyan,Sun, Jing,等. Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice[J]. CELL RESEARCH,2024.
APA	Yan, Bin.,Liu, Chunyan.,Sun, Jing.,Mao, Yang.,Zhou, Can.,...&Cao, Xiaofeng.(2024).Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice.CELL RESEARCH.
MLA	Yan, Bin,et al."Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice".CELL RESEARCH (2024).