Large-Scale Selective Micropatterning with Robotics nDEP Tweezers and Hydrogel Encapsulation

Huang, Kaicheng1,2,3; Lai, Jiewen3,4; Ren, Hongliang4; Wu, Chunhui1,2; Cheng, Xing1,2; Chu, Henry Kar Hang3

2024-09-01

10.1021/acsami.4c10675

ACS APPLIED MATERIALS & INTERFACES   影响因子和分区

1944-8244

1944-8252

Creating diverse microparticle patterns on a large scale enhances the performance and efficiency of biochemical analytics. Current techniques exhibit limitations in achieving diverse microparticle patterns on a large scale, primarily focusing on patterning particles of the same type with limited flexibility and accessibility. Moreover, accessibility to patterned particles without a fixed formation poses additional challenges. Herein, in this work, we introduce a novel robotic micropatterning system designed to address these challenges. The system facilitates the selection, batch transferring, patterning, and encapsulation of microparticles using negative dielectrophoresis (nDEP)-tweezers, enabling large-scale microparticle patterning on a hydrogel. A multielectrode chip was mounted on a micromanipulator to serve as the nDEP tweezers, and the microparticles scattering on the substrate could be trapped and displaced to different positions on a substrate with an array of holes for large-scale pattern generation. Photosensitive hydrogel was employed for microparticle pattern encapsulation. The effects of configuring different experimental parameters on the patterning efficiency were evaluated and analyzed. Experiments were conducted to explore the stability and performance of the micropatterns. Various patterns with hydrogel encapsulation were created using different color polystyrene microbeads (orange, blue, and green) with varying sizes (50, 100, and 125 mu m) under the adjusted environment. Results demonstrate the successful creation of large-scale microbead patterns in a specified form and their encapsulation into an extractable hydrogel using the proposed nDEP tweezer system. The proposed system can be potentially applied to diverse bioparticles for analysis.

automatic control dielectrophoresis micro-and nanoscales micropatterning micromanipulation

SCI

英语

第一

Research Grant Council of the Hong Kong Special Administrative Region[25204016] ; Guangdong-Hong Kong-Macau Joint Laboratory on Micro-Nano Manufacturing Technology[2021LSYS004] ; National Natural Science Foundation of China["62403231","31927802"]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001306493300001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Key Lab Nanoimprint Technol, Shenzhen 518055, Guangdong, Peoples R China
3.Hong Kong Polytech Univ, Dept Mech Engn, Hong Kong 999077, Peoples R China
4.Chinese Univ Hong Kong, Elect Engn Dept, Hong Kong 999077, Peoples R China

Huang, Kaicheng,Lai, Jiewen,Ren, Hongliang,et al. Large-Scale Selective Micropatterning with Robotics nDEP Tweezers and Hydrogel Encapsulation[J]. ACS APPLIED MATERIALS & INTERFACES,2024.

Huang, Kaicheng,Lai, Jiewen,Ren, Hongliang,Wu, Chunhui,Cheng, Xing,&Chu, Henry Kar Hang.(2024).Large-Scale Selective Micropatterning with Robotics nDEP Tweezers and Hydrogel Encapsulation.ACS APPLIED MATERIALS & INTERFACES.

Huang, Kaicheng,et al."Large-Scale Selective Micropatterning with Robotics nDEP Tweezers and Hydrogel Encapsulation".ACS APPLIED MATERIALS & INTERFACES (2024).