Hfq-binding small RNA PqsS regulates Pseudomonasaeruginosa pqs quorum sensing system and virulence

Jia，Tianyuan1,2; Bi，Xianbiao1,2; Li，Menglu1,2; Zhang，Chenhui1,2; Ren，Anmin2; Li，Shangru2; Zhou，Tian2; Zhang，Yingdan1,2; Liu，Yang3; Liu，Xue4; Deng，Yinyue5; Liu，Bin6; Li，Guobao1,2; Yang，Liang1,2

2024-12-01

10.1038/s41522-024-00550-4

npj Biofilms and Microbiomes   影响因子和分区

2055-5008

Pseudomonas aeruginosa is a widespread nosocomial pathogen with a significant to cause both severe planktonic acute and biofilm-related chronic infections. Small RNAs (sRNAs) are noncoding regulatory molecules that are stabilized by the RNA chaperone Hfq to trigger various virulence-related signaling pathways. Here, we identified an Hfq-binding sRNA in P. aeruginosa PAO1, PqsS, which promotes bacterial pathogenicity and pseudomonas quinolone signal quorum sensing (pqs QS) system. Specifically, PqsS enhanced acute bacterial infections by inducing host cell death and promoting rhamnolipid-regulated swarming motility. Meanwhile, PqsS reduced chronic infection traits including biofilm formation and antibiotic resistance. Moreover, PqsS repressed pqsL transcript, increasing PQS levels for pqs QS. A PQS-rich environment promoted PqsS expression, thus forming a positive feedback loop. Furthermore, we demonstrated that the PqsS interacts and destabilizes the pqsL mRNA by recruiting RNase E to drive degradation. These findings provide insights for future research on P. aeruginosa pathogenesis and targeted treatment.

英语

第一 ; 通讯

2-s2.0-85203555579

Scopus

1.Shenzhen National Clinical Research Center for Infectious Disease,Shenzhen Third People’s Hospital,The Second Affiliated Hospital of Southern University of Science and Technology,Shenzhen,China
2.Department of Pharmacology,Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases,School of Medicine,Southern University of Science a-nd Technology,Shenzhen,China
3.Medical Research Center,Southern University of Science and Technology Hospital,Shenzhen,China
4.Department of Pharmacology,Guangdong Key Laboratory for Genome Stability and Human Disease Prevention,International Cancer Center,Shenzhen University Health Science Center,Shenzhen,China
5.School of Pharmaceutical Sciences (Shenzhen),Sun Yat-sen University,Shenzhen,Shenzhen Campus of Sun Yat-sen University,China
6.National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,TEDA Institute of Biological Sciences and Biotechnology,Nankai University,Tianjin,China

Jia，Tianyuan,Bi，Xianbiao,Li，Menglu,et al. Hfq-binding small RNA PqsS regulates Pseudomonasaeruginosa pqs quorum sensing system and virulence[J]. npj Biofilms and Microbiomes,2024,10(1).

Jia，Tianyuan.,Bi，Xianbiao.,Li，Menglu.,Zhang，Chenhui.,Ren，Anmin.,...&Yang，Liang.(2024).Hfq-binding small RNA PqsS regulates Pseudomonasaeruginosa pqs quorum sensing system and virulence.npj Biofilms and Microbiomes,10(1).

Jia，Tianyuan,et al."Hfq-binding small RNA PqsS regulates Pseudomonasaeruginosa pqs quorum sensing system and virulence".npj Biofilms and Microbiomes 10.1(2024).