A ruthenium single atom nanozyme-based antibiotic for the treatment of otitis media caused by Staphylococcus aureus

Wang, Jie1; Gong, Rui1,2; Yang, Ming3; Wu, Xi1; Li, Ziwei4; Huang, Haibing1; Yan, Xiyun1,5,6; Wang, Daji1

2024-08-28

10.3389/fchem.2024.1439039

FRONTIERS IN CHEMISTRY   影响因子和分区

2296-2646

Staphylococcus aureus (S. aureus) infection is a primary cause of otitis media (OM), the most common disease for which children are prescribed antibiotics. However, the abuse of antibiotics has led to a global increase in antimicrobial resistance (AMR). Nanozymes, as promising alternatives to traditional antibiotics, are being extensively utilized to combat AMR. Here, we synthesize a series of single-atom nanozymes (metal-C3N4 SANzymes) by loading four metals (Ag, Fe, Cu, Ru) with antibacterial properties onto a crystalline g-C3N4. These metal-C3N4 display a rob-like morphology and well-dispersed metal atoms. Among them, Ru-C3N4 demonstrates the optimal peroxidase-like activity (285.3 U mg(-1)), comparable to that of horseradish peroxidase (267.7 U mg-1). In vitro antibacterial assays reveal that Ru-C3N4 significantly inhibits S. aureus growth compared with other metal-C3N4 even at a low concentration (0.06 mg mL(-1)). Notably, Ru-C3N4 acts as a narrow-spectrum nanoantibiotic with relative specificity against Gram-positive bacteria. Biofilms formed by S. aureus are easily degraded by Ru-C3N4 due to its high peroxidase-like activity. In vivo, Ru-C(3)N(4 )effectively eliminates S. aureus and relieves ear inflammation in OM mouse models. However, untreated OM mice eventually develop hearing impairment. Due to its low metal load, Ru-C3N4 does not exhibit significant toxicity to blood, liver, or kidney. In conclusion, this study presents a novel SANzyme-based antibiotic that can effectively eliminate S. aureus and treat S. aureus-induced OM.

single atom nanozyme ruthenium peroxidase Staphylococcus aureus otitis media nanoantibiotics

SCI

英语

其他

Chemistry

Chemistry, Multidisciplinary

WOS:001309782400001

FRONTIERS MEDIA SA

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Nanozyme Synth Ctr,Key Lab Quantitat Synthet Biol, Shenzhen, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Fac Synthet Biol, Shenzhen, Peoples R China
3.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp,Affiliated Hosp 1, Clin Med Coll 2,Dept Otolaryngol, Shenzhen, Peoples R China
4.Sun Yat Sen Univ, Shenshan Cent Hosp, Sun Yat Sen Mem Hosp, Dept Clin Lab, Shanwei, Peoples R China
5.Chinese Acad Sci, Inst Biophys, CAS Engn Lab Nanozyme, Key Lab Biomacromol, Beijing, Peoples R China
6.Henan Acad Innovat Med Sci, Nanozyme Lab Zhongyuan, Zhengzhou, Peoples R China

Wang, Jie,Gong, Rui,Yang, Ming,et al. A ruthenium single atom nanozyme-based antibiotic for the treatment of otitis media caused by Staphylococcus aureus[J]. FRONTIERS IN CHEMISTRY,2024,12.

Wang, Jie.,Gong, Rui.,Yang, Ming.,Wu, Xi.,Li, Ziwei.,...&Wang, Daji.(2024).A ruthenium single atom nanozyme-based antibiotic for the treatment of otitis media caused by Staphylococcus aureus.FRONTIERS IN CHEMISTRY,12.

Wang, Jie,et al."A ruthenium single atom nanozyme-based antibiotic for the treatment of otitis media caused by Staphylococcus aureus".FRONTIERS IN CHEMISTRY 12(2024).