Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination

Wang，Zhi Zheng1; Cao，Min Jie2; Yan，Junjie3; Dong，Jin1; Chen，Mo Xian4; Yang，Jing Fang1; Li，Jian Hong4; Ying，Rui Ning1; Gao，Yang Yang4; Li，Li3; Leng，Ya Nan5; Tian，Yuan6; Hewage，Kamalani Achala H.1; Pei，Rong Jie1; Huang，Zhi You1; Yin，Ping3; Zhu，Jian Kang2; Hao，Ge Fei1,4; Yang，Guang Fu1

2024-12-01

10.1038/s41467-024-52426-y

Nature Communications   影响因子和分区

2041-1723

Abscisic acid (ABA) is the primary preventing factor of seed germination, which is crucial to plant survival and propagation. ABA-induced seed germination inhibition is mainly mediated by the dimeric PYR/PYL/RCAR (PYLs) family members. However, little is known about the relevance between dimeric stability of PYLs and seed germination. Here, we reveal that stabilization of PYL dimer can relieve ABA-induced inhibition of seed germination using chemical genetic approaches. Di-nitrobensulfamide (DBSA), a computationally designed chemical probe, yields around ten-fold improvement in receptor affinity relative to ABA. DBSA reverses ABA-induced inhibition of seed germination mainly through dimeric receptors and recovers the expression of ABA-responsive genes. DBSA maintains PYR1 in dimeric state during protein oligomeric state experiment. X-ray crystallography shows that DBSA targets a pocket in PYL dimer interface and may stabilize PYL dimer by forming hydrogen networks. Our results illustrate the potential of PYL dimer stabilization in preventing ABA-induced seed germination inhibition.

英语

其他

2-s2.0-85204010302

Scopus

1.State Key Laboratory of Green Pesticide,International Joint Research Center for Intelligent Biosensor Technology and Health,Central China Normal University,Wuhan,430079,China
2.Institute of Advanced Biotechnology and School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
3.State Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research,Huazhong Agricultural University,Wuhan,430070,China
4.State Key Laboratory of Green Pesticide,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Center for R & amp;D of Fine Chemicals,Guizhou University,Guiyang,550025,China
5.State Key Laboratory of Tree Genetics and Breeding,the Southern Modern Forestry Collaborative Innovation Center,Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation,College of Life Sciences,Nanjing Forestry University,Nanjing,210037,China
6.State Key Laboratory of Crop Biology,College of Life Science,Shandong Agricultural University,Taian,Shandong,271018,China

Wang，Zhi Zheng,Cao，Min Jie,Yan，Junjie,et al. Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination[J]. Nature Communications,2024,15(1).

Wang，Zhi Zheng.,Cao，Min Jie.,Yan，Junjie.,Dong，Jin.,Chen，Mo Xian.,...&Yang，Guang Fu.(2024).Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination.Nature Communications,15(1).

Wang，Zhi Zheng,et al."Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination".Nature Communications 15.1(2024).