Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients

Chen，Na1; Hao，Lilan2,3,4; Zhang，Zhe2; Qin，Chenglu5; Jie，Zhuye2,3; Pan，Hongxin6; Duan，Jiali7; Huang，Xincheng2,8; Zhang，Yunhong9; Gao，Hongqin10; Lu，Ruike10; Sun，Tianshu1; Yang，Hua1; Shi，Jinqiu5; Liang，Maolian5; Guo，Jianbin1; Gao，Qianqian1; Zhao，Xiaoyue1; Dou，Zhiyuan1; Xiao，Liang2,4,11; Zhang，Shaoqiao12; Jin，Xin2; Xu，Xun2; Yang，Huanming2,13; Wang，Jian2,13; Jia，Huijue2,14,15; Zhang，Tao12,16; Kristiansen，Karsten2,3,4; Chen，Chen2,3; Zhu，Lan1

2024-12-01

10.1038/s41467-024-52102-1

Nature Communications   影响因子和分区

2041-1723

Neovaginas are surgically constructed to correct uterovaginal agenesis in women with Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome or as part of gender-affirming surgery for transfeminine individuals. Understanding the assembly of the neovaginal microbiota is crucial for guiding its management. To address this, we conducted a longitudinal study on MRKH patients following laparoscopic peritoneal vaginoplasty. Our findings reveal that the early microbial assemblage exhibited stochastic characteristics, accompanied with a notable bloom of Enterococcus faecalis and genital Mycoplasmas. While both the pre-surgery dimple microbiota and the fecal microbiota constituted the primary species pool, the neovaginal microbiota developed into a microbiota that resembled that of a normal vagina at 6–12 months post-surgery, albeit with a bacterial vaginosis (BV)-like structure. By 2–4 years post-surgery, the neovaginal microbiota had further evolved into a structure closely resembling with the homeostatic pre-surgery dimple microbiota. This concords with the development of the squamous epithelium in the neovagina and highlights the pivotal roles of progressive selective forces imposed by the evolving neovaginal environment and the colonization tropism of vaginal species. Notably, we observed that strains of Lactobacillus crispatus colonizing the neovagina primarily originated from the dimple. Since L. crispatus is generally associated with vaginal health, this finding suggests potential avenues for future research to promote its colonization.

英语

其他

2-s2.0-85203243936

Scopus

1.National Clinical Research Center for Obstetric and Gynecologic Diseases,Department of Obstetrics and Gynecology,Peking Union Medical College Hospital,State Key Laboratory for Complex Severe and Rare Diseases,Peking Union Medical College,Chinese Academy of Medical Sciences,No. 1 Shuaifuyuan,Beijing,100005,China
2.BGI-Research,Shenzhen,518083,China
3.Department of Biology,Ole Maaløes Vej 5,University of Copenhagen,Copenhagen,DK-2200,Denmark
4.Qingdao-Europe Advanced Institute for Life Sciences,BGI-Research,Qingdao,266555,China
5.Department of Obstetrics and Gynaecology,The 3rd Affiliated Hospital of Shenzhen University,Luohu hospital,Shenzhen,Guangdong,518000,China
6.Department of Gynecology,Southern University of Science and Technology Hospital,Shenzhen,6019 liuxian street,518000,China
7.Chinese Academy of Medical Sciences & amp; Peking Union Medical College,4+4 Medical Doctor Program,No.9 Dongdansantiao,Beijing,100730,China
8.China National GeneBank,BGI-Research,Shenzhen,518210,China
9.Social Affairs Bureau of Suzhou National New and Hi-tech Industrial Development Zone,Suzhou,215163,China
10.Suzhou National New and Hi-tech Industrial Development Zone Center for Maternal and Child Health and Family Planning Service,Suzhou,215163,China
11.Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome,BGI-Research,Shenzhen,518083,China
12.BGI Research,Wuhan,430074,China
13.James D. Watson Institute of Genome Sciences,Hangzhou,310013,China
14.School of Life Sciences,Fudan University,Shanghai,200433,China
15.Greater Bay Area Institute of Precision Medicine (Guangzhou),Fudan University,Guangzhou,511458,China
16.Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research,Shenzhen,Guangdong,518083,China

Chen，Na,Hao，Lilan,Zhang，Zhe,et al. Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients[J]. Nature Communications,2024,15(1).

Chen，Na.,Hao，Lilan.,Zhang，Zhe.,Qin，Chenglu.,Jie，Zhuye.,...&Zhu，Lan.(2024).Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients.Nature Communications,15(1).

Chen，Na,et al."Insights into the assembly of the neovaginal microbiota in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome patients".Nature Communications 15.1(2024).