南方科技大学知识苑(SUSTech KC): High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples

题名	High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples
作者	Zhou, Lulu; Shi, Wei; Fu, Shuang; Li, Mengfan ; Chen, Jianwei ; Fang, Ke; Li, Yiming
通讯作者	Li, Yiming
发表日期	2024-09-01
DOI	10.1021/acs.analchem.4c02893
发表期刊	ANALYTICAL CHEMISTRY 影响因子和分区
ISSN	0003-2700
EISSN	1520-6882
摘要	The current limitations of single-molecule localization microscopy (SMLM) in deep tissue imaging, primarily due to depth-dependent aberrations caused by refractive index (RI) mismatch, present a significant challenge in achieving high-resolution images at greater depths. To extend the imaging depth, we optimized the imaging buffer of SMLM with the RI matched to that of the objective immersion medium and systematically evaluated five different RI-matched buffers, focusing on their impact on the blinking behavior of red-absorbing dyes and the quality of reconstructed super-resolution images. Particularly, we found that clear unobstructed brain imaging cocktails-based imaging buffer could match the RI of oil and was able to clear the tissue samples. With the help of the RI-matched imaging buffers, we showed high-quality dual-color 3D SMLM images with imaging depths ranging from a few micrometers to tens of micrometers in both cultured cells and sectioned tissue samples. This advancement offers a practical and accessible method for high-resolution imaging at greater depths without the need for specialized optical equipment or expertise.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China[62375116] ; Shenzhen Medical Research Fund[B2302038] ; Key Technology Research and Development Program of Shandong[2021CXGC010212] ; Shenzhen Science and Technology Innovation Commission["JCYJ20220818100416036","KQTD20200820113012029"] ; Guangdong Natural Science Foundation Joint Fund[2024A1515011565] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Postdoctoral Fellowship Program of CPSF[GZC20240651] ; Postdoctoral Research grant for Retaining (or Recruiting) Talent in Shenzhen[K24387511]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:001318495200001
出版者	AMER CHEMICAL SOC
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/834193
专题	工学院_生物医学工程系
作者单位	Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Zhou, Lulu,Shi, Wei,Fu, Shuang,et al. High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples[J]. ANALYTICAL CHEMISTRY,2024.
APA	Zhou, Lulu.,Shi, Wei.,Fu, Shuang.,Li, Mengfan.,Chen, Jianwei.,...&Li, Yiming.(2024).High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples.ANALYTICAL CHEMISTRY.
MLA	Zhou, Lulu,et al."High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples".ANALYTICAL CHEMISTRY (2024).