A Surface-Mediated Biomimetic Porous Polyether-Ether-Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis

Zhu, Mingyu1,4; Hu, Liqiu2; Liu, Yang2; Chen, Pinghang1; Wang, Xiaofei1; Tang, Bin2; Liu, Chao2; Zhang, Rui3; Fang, Ju1; Ren, Fuzeng1

2024-09-01

10.1021/acsbiomaterials.4c00725

ACS BIOMATERIALS SCIENCE & ENGINEERING   影响因子和分区

2373-9878

The repair of critical-sized bone defects remains a major challenge for clinical orthopedic surgery. Here, we develop a surface biofunctionalized three-dimensional (3D) porous polyether-ether-ketone (PEEK) scaffold that can simultaneously promote osteogenesis and regulate macrophage polarization. The scaffold is created using polydopamine (PDA)-assisted immobilization of silk fibroin (SF) and the electrostatic self-assembly of nanocrystalline hydroxyapatite (nano-HA) on a 3D-printed porous PEEK scaffold. The SF/nano-HA functionalized surface provides a bone-like microenvironment for osteoblastic cells' adhesion, proliferation, mineralization and osteogenic differentiation. Moreover, the biofunctionalized surface can effectively drive macrophages polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype. Integrin beta 1-specific cell-matrix binding and the activation of Ca2+ receptor-mediated signaling pathway play critical roles in the regulation of macrophage polarization. Compared with the as-printed scaffold, the SF/nano-HA functionalized porous PEEK scaffold induces minimal inflammatory response, enhanced angiogenesis, and substantial new bone formation, resulting in improved osseointegration in vivo. This study not only develops a promising candidate for bone repair but also demonstrates a facile surface biofunctionalization strategy for orthopedic implants to improve osseointegration by stimulating osteogenesis and regulating immunity.

porous PEEK surface modification osteogenicdifferentiation macrophage polarization osseointegration

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[52122102] ; Fundamental and Applied Fundamental Research Fund of Guangdong Province, China[2022B1515120082]

Materials Science

Materials Science, Biomaterials

WOS:001317056000001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Stomatol Ctr, Dept Prosthodont, Shenzhen 518036, Guangdong, Peoples R China
4.Univ Helsinki, Fac Pharm, Div Pharmaceut Chem & Technol, Helsinki 00790, Finland

Zhu, Mingyu,Hu, Liqiu,Liu, Yang,et al. A Surface-Mediated Biomimetic Porous Polyether-Ether-Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2024.

Zhu, Mingyu.,Hu, Liqiu.,Liu, Yang.,Chen, Pinghang.,Wang, Xiaofei.,...&Ren, Fuzeng.(2024).A Surface-Mediated Biomimetic Porous Polyether-Ether-Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis.ACS BIOMATERIALS SCIENCE & ENGINEERING.

Zhu, Mingyu,et al."A Surface-Mediated Biomimetic Porous Polyether-Ether-Ketone Scaffold for Regulating Immunity and Promoting Osteogenesis".ACS BIOMATERIALS SCIENCE & ENGINEERING (2024).