Molecular serotyping of diarrheagenic Escherichia coli with a MeltArray assay reveals distinct correlation between serotype and pathotype

Du, Chen1,2,3; Liao, Yiqun1,2; Ding, Congcong1,2; Huang, Jiayu1,2; Zhou, Shujuan1,2; Xu, Yiyan1,2; Yang, Zhaohui1,2; Shi, Xiaolu3; Li, Yinghui3; Jiang, Min3; Zuo, Le3; Li, Minxu3; Bian, Shengzhe4; Xiao, Na5; Li, Liqiang6; Xu, Ye1,2; Hu, Qinghua3; Li, Qingge1,2

2024-12-31

10.1080/19490976.2024.2401944

GUT MICROBES   影响因子和分区

1949-0976

1949-0984

Diarrheagenic Escherichia coli serotypes are associated with various clinical syndromes, yet the precise correlation between serotype and pathotype remains unclear. A major barrier to such studies is the reliance on antisera-based serotyping, which is culture-dependent, low-throughput, and cost-ineffective. We have established a highly multiplex PCR-based serotyping assay, termed the MeltArray E. coli serotyping (EST) assay, capable of identifying 163 O-antigen-encoding genes and 53 H-antigen-encoding genes of E. coli. The assay successfully identified serotypes directly from both simulated and real fecal samples, as demonstrated through spike-in validation experiments and a retrospective study. In a multi-province study involving 637 E. coli strains, it revealed that the five major diarrheagenic pathotypes have distinct serotype compositions. Notably, it differentiated 257 Shigella isolates into four major Shigella species, distinguishing them from enteroinvasive E. coli based on their distinct serotype profiles. The assay's universality was further corroborated by in silico analysis of whole-genome sequences from the EnteroBase. We conclude that the MeltArray EST assay represents a paradigm-shifting tool for molecular serotyping of E. coli, with potential routine applications for comprehensive serotype analysis, disease diagnosis, and outbreak detection.

Diarrheagenic Escherichia coli Shigella molecular serotyping culture-independent serotyping correlation between serotype and pathotype

SCI

英语

其他

Important National Science and Technology Specific Project[2017ZX10303406] ; National Key R&D Program of China["KY202002008","2021YFC1200200"] ; Major Science and Technology Project of the Inner Mongolia Autonomous Region[2021ZD0006] ; Fujian University Industry-University-Research Joint Innovation Project[2021Y4001] ; Xiamen Major Science and Technology Project[3502Z20191007] ; Shenzhen Science and Technology Program[KCXFZ20230731093959008] ; Sanming Project of Medicine in Shenzhen[SZSM202311015]

Gastroenterology & Hepatology ; Microbiology

Gastroenterology & Hepatology ; Microbiology

WOS:001315692700001

TAYLOR & FRANCIS INC

Web of Science

1.Xiamen Univ, Engn Res Ctr Mol Diagnost, Sch Life Sci, Minist Educ,State Key Lab Cellular Stress Biol,Sta, 4221 South Xiangan Rd, Xiamen 361102, Peoples R China
2.Xiamen Univ, Sch Publ Hlth, Xiamen, Peoples R China
3.Shenzhen Ctr Dis Control & Prevent, Microbiol Lab Off, Shenzhen, Peoples R China
4.BGI Shenzhen, GI Res, Shenzhen, Peoples R China
5.Yantian Dist Ctr Dis Control & Prevent, Lab Dept, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China

Du, Chen,Liao, Yiqun,Ding, Congcong,et al. Molecular serotyping of diarrheagenic Escherichia coli with a MeltArray assay reveals distinct correlation between serotype and pathotype[J]. GUT MICROBES,2024,16(1).

Du, Chen.,Liao, Yiqun.,Ding, Congcong.,Huang, Jiayu.,Zhou, Shujuan.,...&Li, Qingge.(2024).Molecular serotyping of diarrheagenic Escherichia coli with a MeltArray assay reveals distinct correlation between serotype and pathotype.GUT MICROBES,16(1).

Du, Chen,et al."Molecular serotyping of diarrheagenic Escherichia coli with a MeltArray assay reveals distinct correlation between serotype and pathotype".GUT MICROBES 16.1(2024).