"Multi-layer" encryption of medical data in DNA for highly-secure storage

Xu, Jiaxin1,2; Wang, Yu2; Chen, Xue2; Wang, Lingwei1; Zhou, Haibo3; Mei, Hui2; Chen, Shanze1; Huang, Xiaoluo2

2024-10-01

10.1016/j.mtbio.2024.101221

MATERIALS TODAY BIO   影响因子和分区

2590-0064

The exponential increasement and the attributes of medical data drive the requirement for secure medical data archiving. DNA data storage shows promise for storing sensitive and important data like medical records due to its high density and endurance. Nevertheless, current DNA data storage working scheme generally does not fully consider the data encryption, posing a risk of data corruption by routine DNA sequencing. Here, we designed a "multi-layer" encryption pipeline for medical data archiving. Initially, digital information was encrypted using Blowfish algorithm at information technology (IT) layer, followed by two-layer data encryption at the biotechnology (BT) layer. The first BT layer exploited the molecular weight of synthetic DNA or nucleoside to encrypt the key, while the second BT layer encrypted digital information within DNA sequences. Consequently, decryption involved layer-by-layer interpretation of data, including mass spectroscopy, sequencing, and Blowfish decryption, significantly enhancing data security. Utilizing mass spectroscopy to retrieve information allows for employment of both natural and unnatural nucleosides, as well as their synthetic oligonucleotides, for data storage, thereby considerably boosting scalability. Our work implies expanded flexibility of DNA-based data storage, highlighting the potential for leveraging various physical and chemical characteristics of DNA molecules to encode and access digital information.

Medical data Data security DNA data storage "Multi-layer" encryption Mass spectroscopy

SCI

英语

第一 ; 通讯

National Key Research and Development Program of China[2021YFF1201700] ; National Natural Science Foundation of China["32201207","32100734"] ; Shenzhen Medical Academy of Research and Translation["C2302001","B2302041"] ; Guangdong Basic and Applied Basic Research Foundation[2214050008970] ; Shenzhen Science Technology and Innovative Commission[KCXFZ202002011008256] ; Shenzhen Science and Technology Program[RCYX20221008092950122]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:001314763200001

ELSEVIER

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Dept Pulm & Crit Care Med,Inst Resp Dis,Postdoctor, Shenzhen 518020, Guangdong, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom,Guangdong Prov Key, Shenzhen 518055, Guangdong, Peoples R China
3.Jinan Univ, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China

Xu, Jiaxin,Wang, Yu,Chen, Xue,et al. "Multi-layer" encryption of medical data in DNA for highly-secure storage[J]. MATERIALS TODAY BIO,2024,28.

Xu, Jiaxin.,Wang, Yu.,Chen, Xue.,Wang, Lingwei.,Zhou, Haibo.,...&Huang, Xiaoluo.(2024)."Multi-layer" encryption of medical data in DNA for highly-secure storage.MATERIALS TODAY BIO,28.

Xu, Jiaxin,et al.""Multi-layer" encryption of medical data in DNA for highly-secure storage".MATERIALS TODAY BIO 28(2024).