Renal-Clearable Organic Probes From D-A-D Type Aza-BODIPY Fluorophores for Multiphoton Deep-Brain Imaging

Li, Wenting1,2; Deng, Xiangquan3; Zhang, Zhe4; Fang, Xiaofeng1; Liu, Ye1; Yang, Yicheng1; Zhong, Jicheng3; Zhang, Chi3; Zhang, Yingxian3; Wang, Yingjie5; Wu, Changfeng1; Wang, Ke3

2024-09-01

10.1002/smll.202403994

SMALL   影响因子和分区

1613-6810

1613-6829

["Bright near-infrared (NIR) fluorescent probes play an important role in in vivo optical imaging. Here, renal-clearable nanodots prepared from Aza-BODIPY are reported fluorophores for multiphoton brain imaging. The design of donor-acceptor-donor (D-A-D) type conjugated structures endowed the fluorophores with large three-photon absorption cross-section for both 1620 and 2200 nm excitation. The side chain modification and lipid encapsulation yield ultrasmall nanodots (approximate to 4 nm) and a high fluorescence quantum yield (approximate to 0.35) at 720 nm emission in the aqueous phase. The measured three-photon action cross-section of a single Aza-BODIPY fluorophore in the nanodots is approximate to 30 times higher than the commonly used Sulforhodamine 101 dye. Three-photon deep brain imaging of subcortical structures is demonstrated, reaching a depth of 1900 mu m below the brain surface in a live mouse study. The nanodots enabled blood flow measurement at a depth of 1617 mu m using line scanning three-photon microscopy (3PM). This work provides superior fluorescent probes for multiphoton deep-brain imaging.","Renal-clearable nanodots prepared from D-A-D type Aza-BODIPY fluorophores exhibit ultra-small size (approximate to 4 nm), high fluorescence quantum yield (approximate to 0.35) at 720 nm emission in aqueous phase, and three-photon action cross-section 30 times higher than Rhodamine dyes. The brain vasculature down to a depth of 1900 mu m can be clearly resolved after craniotomy in mouse study. image"]

Aza-BODIPY fluorophores D-A-D type conjugated structures multiphoton brain imaging renal-clearable nanodots

SCI

英语

其他

Shenzhen Science and Technology Innovation Program["KQTD20170810111314625","JCYJ20210324115807021","SGDX20211123114002003"] ; Shenzhen Bay Laboratory[SZBL2021080601002] ; Guangdong-Hong Kong Technology Cooperation Funding Scheme[GHP/176/21SZ] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Shenzhen Key Laboratory of Photonics and Biophotonics[ZDSYS20210623092006020] ; Guangdong Basic and Applied Basic Research Foundation[2024A1515013285] ; null[62235007] ; null[22204070] ; null[61975126]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:001322261800001

WILEY-V C H VERLAG GMBH

Web of Science

1.Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
3.Shenzhen Bay Lab, Shenzhen 518132, Guangdong, Peoples R China
4.Guangdong Med Univ, Dongguan Affiliated Hosp 1, Guangdong Prov Key Lab Med Immunol & Mol Diag, Sch Med Technol, Dongguan 523808, Peoples R China
5.Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, Shenzhen 518118, Peoples R China

Li, Wenting,Deng, Xiangquan,Zhang, Zhe,et al. Renal-Clearable Organic Probes From D-A-D Type Aza-BODIPY Fluorophores for Multiphoton Deep-Brain Imaging[J]. SMALL,2024.

Li, Wenting.,Deng, Xiangquan.,Zhang, Zhe.,Fang, Xiaofeng.,Liu, Ye.,...&Wang, Ke.(2024).Renal-Clearable Organic Probes From D-A-D Type Aza-BODIPY Fluorophores for Multiphoton Deep-Brain Imaging.SMALL.

Li, Wenting,et al."Renal-Clearable Organic Probes From D-A-D Type Aza-BODIPY Fluorophores for Multiphoton Deep-Brain Imaging".SMALL (2024).