A novel triple-signal biosensor based on ZrFe-MOF@PtNPs for ultrasensitive aflatoxins detection

Sun, Boyan1; Panferov, Vasily2; Guo, Xuewen2; Xiong, Jincheng3; Zhang, Shuai1; Qin, Linqian1; Yin, Chunchao1; Wang, Xiaomei1; Liu, Chujun1; Han, Ke1; Wang, Sihan2; Jiang, Haiyang1

2025

10.1016/j.bios.2024.116797

BIOSENSORS & BIOELECTRONICS   影响因子和分区

0956-5663

1873-4235

The development of more sensitive, stable, and portable biosensors is crucial for meeting the growing demands of diverse and complex detection environments. MOF-based nanozymes have emerged as excellent optical reporters, making them ideal signal donors for constructing multi-signal lateral flow immunoassays (LFIA). In this study, a ZrFe-MOF@PtNPs nanocomposite was synthesized by uniformly depositing platinum nanoparticles (PtNPs) onto the surface of ZrFe-MOFs using an impregnation-reduction method. The ZrFe-MOF@PtNPs exhibited broad absorption spectra, excellent peroxidase-like activity (SA = 21.77 U/mg), high solvent stability, and efficient antibody binding capability. A portable LFIA platform was developed based on ZrFe-MOF@PtNPs and a smartphone for the targeted detection of carcinogenic aflatoxins. This method enabled the readout of colorimetric, fluorescent, and catalytic signals, significantly enhancing detection sensitivity, ensuring result accuracy, and expanding the dynamic detection range. For aflatoxin M1, the calculation of the detection limit of the three signal modes reached as low as 0.0062 ng/mL, which is two orders of magnitude more sensitive than AuNPs-LFIA (0.1839 ng/mL). This study provides effective guidance for multifunctional modification of MOFs and serves as a reference for the application of MOF-based nanozymes in point-of-care biosensors.

Metal-organic frameworks Platinum nanoparticles Nanozyme Multiplex lateral flow immunoassay Aflatoxins

SCI

英语

其他

National Natural Science Foun-dation of China[32373096]

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology

WOS:001321180100001

ELSEVIER ADVANCED TECHNOLOGY

Web of Science

1.China Agr Univ, Natl Key Lab Vet Publ Hlth Secur, Beijing Key Lab Detect Technol Anim Derived Food S, Beijing Lab Food Qual & Safety,Coll Vet Med, Beijing 100193, Peoples R China
2.Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada
3.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen Key Lab Smart Healthcare Engn, Shenzhen 518055, Guangdong, Peoples R China

Sun, Boyan,Panferov, Vasily,Guo, Xuewen,et al. A novel triple-signal biosensor based on ZrFe-MOF@PtNPs for ultrasensitive aflatoxins detection[J]. BIOSENSORS & BIOELECTRONICS,2025,267.

Sun, Boyan.,Panferov, Vasily.,Guo, Xuewen.,Xiong, Jincheng.,Zhang, Shuai.,...&Jiang, Haiyang.(2025).A novel triple-signal biosensor based on ZrFe-MOF@PtNPs for ultrasensitive aflatoxins detection.BIOSENSORS & BIOELECTRONICS,267.

Sun, Boyan,et al."A novel triple-signal biosensor based on ZrFe-MOF@PtNPs for ultrasensitive aflatoxins detection".BIOSENSORS & BIOELECTRONICS 267(2025).