Microglia inversely regulate the level of perineuronal nets with the treatment of lipopolysaccharide and valproic acid

Liu, Luping1,2; Li, Tianxiang2; Chang, Jinlong3; Xia, Xiaojiao2; Ju, Jun2,4,5

2024-11-01

10.1016/j.neulet.2024.137992

NEUROSCIENCE LETTERS   影响因子和分区

0304-3940

1872-7972

Perineuronal nets (PNNs) are extracellular matrix which mostly surround the inhibitory neurons. They are changed in several brain diseases, such as autism spectrum disorder, but the mechanism of PNNs degradation is still unclear. In this study, we investigated the role of microglial cells in regulating PNNs levels. Specifically, 1 day or 3 days after a single dose of lipopolysaccharide (LPS, 0.25 mg/kg) increased the density of microglia and further reduced the density of PNNs in both hippocampus CA1 and visual cortex. Minocycline, an inhibitor of microglia activation, took effect time-dependently. Minocycline for 7 days before a single LPS injection (0.25 mg/kg) inhibited microglia increase and PNNs loss, but minocycline for 3 days did not work. Finally, in a valproic acid (VPA)-treated autism mouse model, microglia were reduced while PNNs(+) cells were increased in both hippocampus CA1 and visual cortex. In summary, the microglia are involved in the balanced level of PNNs, while in the autism model, the altered level of PNNs might be due to the microglia hypofunction.

Perineuronal nets Microglia Lipopolysaccharide Minocycline Autism spectrum disorder

SCI

英语

通讯

National Natural Science Foundation of China[82301730]

Neurosciences & Neurology

Neurosciences

WOS:001321615600001

ELSEVIER IRELAND LTD

Web of Science

1.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong, Peoples R China
2.Peking Univ, Sch Chem Biol & Biotechnol, Shenzhen Grad Sch, Shenzhen, Peoples R China
3.Dali Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Dali 671003, Peoples R China
4.Southern Univ Sci & Technol, Brain Res Ctr, Shenzhen, Peoples R China
5.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China

Liu, Luping,Li, Tianxiang,Chang, Jinlong,et al. Microglia inversely regulate the level of perineuronal nets with the treatment of lipopolysaccharide and valproic acid[J]. NEUROSCIENCE LETTERS,2024,842.

Liu, Luping,Li, Tianxiang,Chang, Jinlong,Xia, Xiaojiao,&Ju, Jun.(2024).Microglia inversely regulate the level of perineuronal nets with the treatment of lipopolysaccharide and valproic acid.NEUROSCIENCE LETTERS,842.

Liu, Luping,et al."Microglia inversely regulate the level of perineuronal nets with the treatment of lipopolysaccharide and valproic acid".NEUROSCIENCE LETTERS 842(2024).