Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA

Zhong, Wukun1; Geng, Chunyang2; Fu, Zhangcheng1; Mao, Cuiping2; Zheng, Yanlin1; Wang, Saijie2; Liu, Kai3; Yang, Yang4,5; Lu, Chunhua1; Jiang, Xingyu2

2024-09-01

10.1021/acs.analchem.4c04637

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

As digital data undergo explosive growth, deoxyribonucleic acid (DNA) has emerged as a promising storage medium due to its high density, longevity, and ease of replication, offering vast potential in data storage solutions. This study focuses on the protection and retrieval of data during the DNA storage process, developing a technique that employs flow cytometry sorting (FCS) to segregate multicolored fluorescent DNA microparticles encoded with data and facilitating efficient random access. Moreover, the encapsulated fluorescent DNA microparticles, formed through layer-by-layer self-assembly, preserve structural and sequence integrity even under harsh conditions while also supporting a high-density DNA payload. Experimental results have shown that the encoded data can still be successfully recovered from encapsulated DNA microparticles following de-encapsulation. We also successfully demonstrated the automated encapsulation process of fluorescent DNA microparticles using a microfluidic chip. This research provides an innovative approach to the long-term stability and random readability of DNA data storage.

SCI

英语

通讯

National Key R&D Program of China[2018YFA0902600] ; National Natural Science Foundation of China[22174019] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; Shenzhen Science and Technology Program[KQTD20190929172743294] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Guangdong Major Talent Introduction Project[2019CX01Y196]

Chemistry

Chemistry, Analytical

WOS:001320389600001

AMER CHEMICAL SOC

Web of Science

1.Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350108, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China
3.Tsinghua Univ, Engn Res Ctr Adv Rare Earth Mat, Dept Chem, Minist Educ, Beijing 100084, Peoples R China
4.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med, Shanghai 200127, Peoples R China
5.Shanghai Jiao Tong Univ, Shanghai Key Lab Nucle Acid Chem & Nanomed, State Key Lab Oncogenes & Related Genes, Renji Hosp,Sch Med, Shanghai 200127, Peoples R China

Zhong, Wukun,Geng, Chunyang,Fu, Zhangcheng,et al. Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA[J]. ANALYTICAL CHEMISTRY,2024.

Zhong, Wukun.,Geng, Chunyang.,Fu, Zhangcheng.,Mao, Cuiping.,Zheng, Yanlin.,...&Jiang, Xingyu.(2024).Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA.ANALYTICAL CHEMISTRY.

Zhong, Wukun,et al."Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA".ANALYTICAL CHEMISTRY (2024).