Immunotoxic response of bio-based plastic on early life stage zebrafish (Danio rerio): A safe alternative to petroleum-based plastics?

Cheng, Haodong1; Zou, Yao2,3; Lu, Bin4; Wang, Jiazhen5,6; Magnuson, Jason T.7; Xu, Bentuo8; Qiu, Wenhui5,6; Xuan, Rongrong1

2024-12-05

10.1016/j.jhazmat.2024.135846

JOURNAL OF HAZARDOUS MATERIALS   影响因子和分区

0304-3894

1873-3336

induce immunotoxic responses in fish. Our research focused on the accumulation and immunotoxicity of 80 nm polylactic acid (PLA) and polystyrene (PS) (0.1-10 mg/L) on early life stage zebrafish (Danio rerio) exposed for 7 days. Compared to PS, there was a higher accumulation of PLA in larvae. Exposure to PLA resulted in a significant increase in neutrophils and macrophages, while immune protein levels such as Complement 3 (C3), Immunoglobulin M (IgM), and C-reactive protein (CRP) were significantly reduced. Furthermore, the mRNA expression of pro-inflammatory cytokines, including tnf-alpha and il-6, were significantly elevated in PLA treatments. Additionally, PLA-exposed zebrafish were more susceptible to infection by Vibrio parahaemolyticus. Interestingly, at the same concentration, exposures to PS did not induce significant changes in macrophages or immune protein levels, C3 and IgM. This suggests that PLA has a greater immunotoxic response relative to PS. Our research findings contradict the popular belief that bio-based plastics are non-toxic and harmless, which may have potential risk to aquatic organisms.

Bio-based plastics Petroleum-based plastics Zebrafish Antibacterial ability Immunotoxicity

SCI

英语

通讯

National Natural Science Foundation of China["42322707","42277266"] ; Shenzhen Science and Technology Innovation Committee[20220814233432001] ; Ningbo key research and development plan and "reveal the list" project[2023Z175] ; Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control[2023B1212060002] ; Guangdong-Hong Kong Joint Laboratory for Soil and Groundwater Pollution Control[2023B1212120001] ; High level of special funds[G030290001]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:001320224000001

ELSEVIER

Web of Science

1.Ningbo Univ, Affiliated Hosp 1, Ningbo 315020, Peoples R China
2.South China Univ Technol, Sch Environm & Energy, Guangzhou 510006, Peoples R China
3.Guangdong Soc Environm Sci, Guangzhou 510045, Peoples R China
4.Shanghai Univ, Sch Environm & Chem Engn, Key Lab Organ Cpd Pollut Control Engn, MOE, Shanghai 200444, Peoples R China
5.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China
6.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Hong Kong Joint Lab Soil & Groundwater P, 1088 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China
7.US Geol Survey, Columbia Environm Res Ctr, Columbia, MO 65201 USA
8.Wenzhou Univ, Natl & Local Joint Engn Res Ctr Ecol Treatment Tec, Sch Life & Environm Sci, Wenzhou 325035, Peoples R China

Cheng, Haodong,Zou, Yao,Lu, Bin,et al. Immunotoxic response of bio-based plastic on early life stage zebrafish (Danio rerio): A safe alternative to petroleum-based plastics?[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,480.

Cheng, Haodong.,Zou, Yao.,Lu, Bin.,Wang, Jiazhen.,Magnuson, Jason T..,...&Xuan, Rongrong.(2024).Immunotoxic response of bio-based plastic on early life stage zebrafish (Danio rerio): A safe alternative to petroleum-based plastics?.JOURNAL OF HAZARDOUS MATERIALS,480.

Cheng, Haodong,et al."Immunotoxic response of bio-based plastic on early life stage zebrafish (Danio rerio): A safe alternative to petroleum-based plastics?".JOURNAL OF HAZARDOUS MATERIALS 480(2024).