南方科技大学知识苑(SUSTech KC): Highly sensitive site-specific SUMOylation proteomics in <i>Arabidopsis</i>

题名	Highly sensitive site-specific SUMOylation proteomics in Arabidopsis
作者	Sang, Tian1,2 ; Xu, Yaping 3,6; Qin, Guochen 4; Zhao, Shasha 1,2; Hsu, Chuan-Chi 5; Wang, Pengcheng1,2
通讯作者	Wang, Pengcheng
发表日期	2024-09-01
DOI	10.1038/s41477-024-01783-z
发表期刊	NATURE PLANTS 影响因子和分区
ISSN	2055-026X
EISSN	2055-0278
卷号	10 期号:9
摘要	SUMOylation-the attachment of a small ubiquitin-like modifier (SUMO) to target proteins-plays roles in controlling plant growth, nutrient signalling and stress responses. SUMOylation studies in plants are scarce because identifying SUMOylated proteins and their sites is challenging. To date, only around 80 SUMOylation sites have been identified. Here we introduce lysine-null SUMO1 into the Arabidopsissumo1 sumo2 mutant and establish a two-step lysine-null SUMO enrichment method. We identified a site-specific SUMOylome comprising over 2,200 SUMOylation sites from 1,300 putative acceptors that function in numerous nuclear processes. SUMOylation marks occur on several motifs, differing from the canonical psi KxE motif in distant eukaryotes. Quantitative comparisons demonstrate that SUMOylation predominantly enhances the stability of SUMO1 acceptors. Our study delivers a highly sensitive and efficient method for site-specific SUMOylome studies and provides a comprehensive catalogue of Arabidopsis SUMOylation, serving as a valuable resource with which to further explore how SUMOylation regulates protein function.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Key Research and Development Program of China, Grant 2021YFA1300402[2021YFA1300402]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001315881600016
出版者	NATURE PORTFOLIO
来源库	Web of Science
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/834333
专题	南方科技大学医学院_前沿生物技术研究院南方科技大学医学院
作者单位	1.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China 2.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China 4.Peking Univ, Shandong Lab Adv Agr Sci Weifang, Inst Adv Agr Sci, Weifang, Peoples R China 5.Acad Sinica, Inst Plant & Microbial Biol, Taipei, Taiwan 6.Anhui Agr Univ, Sch Life Sci, Natl Engn Lab Crop Stress Resistance Breeding, Hefei, Peoples R China
第一作者单位	前沿生物技术研究院; 南方科技大学医学院
通讯作者单位	前沿生物技术研究院; 南方科技大学医学院
第一作者的第一单位	前沿生物技术研究院
推荐引用方式 GB/T 7714	Sang, Tian,Xu, Yaping,Qin, Guochen,et al. Highly sensitive site-specific SUMOylation proteomics in Arabidopsis[J]. NATURE PLANTS,2024,10(9).
APA	Sang, Tian,Xu, Yaping,Qin, Guochen,Zhao, Shasha,Hsu, Chuan-Chi,&Wang, Pengcheng.(2024).Highly sensitive site-specific SUMOylation proteomics in Arabidopsis.NATURE PLANTS,10(9).
MLA	Sang, Tian,et al."Highly sensitive site-specific SUMOylation proteomics in Arabidopsis".NATURE PLANTS 10.9(2024).