Strain-level multidrug-resistant pathogenic bacteria in urban wastewater treatment plants: Transmission, source tracking and evolution

Zhao，Yanmei1; Fan，Lu3,4; Gao，Shu Hong1; Huang，Fang2; Lei，Zelin3; Wang，Wenxiu3; Gao，Rui2; Dai，Zihan1; Li，Qian1; Liang，Bin1; Wang，Aijie1

2024-12-01

10.1016/j.watres.2024.122538

Water Research   影响因子和分区

0043-1354

1879-2448

Wastewater treatment plants (WWTPs) serve as reservoirs for various pathogens and play a pivotal role in safeguarding environmental safety and public health by mitigating pathogen release. Pathogenic bacteria, known for their potential to cause fatal infections, present a significant and emerging threat to global health and remain poorly understood regarding their origins and transmission in the environment. Using metagenomic approaches, we identified a total of 299 pathogens from three full-scale WWTPs. We comprehensively elucidated the occurrence, dissemination, and source tracking of the pathogens across the WWTPs, addressing deficiencies in traditional detection strategies. While indicator pathogens in current wastewater treatment systems such as Escherichia coli are effectively removed, specific drug-resistant pathogens, including Pseudomonas aeruginosa, Pseudomonas putida, and Aeromonas caviae, persist throughout the treatment process, challenging complete eradication efforts. The anoxic section plays a predominant role in controlling abundance but significantly contributes to downstream pathogen diversity. Additionally, evolution throughout the treatment process enhances pathogen diversity, except for upstream transmission, such as A. caviae str. WP8-S18-ESBL-04 and P. aeruginosa PAO1. Our findings highlight the necessity of expanding current biomonitoring indicators for wastewater treatment to optimize treatment strategies and mitigate the potential health risks posed by emerging pathogens. By addressing these research priorities, we can effectively mitigate risks and safeguard environmental safety and public health.

Drug-resistant pathogens Metagenomics source tracking Strain-level evolution Wastewater treatment plants

英语

其他

2-s2.0-85205310844

Scopus

1.State Key Laboratory of Urban Water Resource and Environment,School of Civil & Environmental Engineering,Harbin Institute of Technology Shenzhen,Shenzhen,518055,China
2.State Key Laboratory of Urban Water Resource and Environment,School of Environment,Harbin Institute of Technology,Harbin,150090,China
3.Department of Ocean Science and Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,China
4.Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou),Guangzhou,China

Zhao，Yanmei,Fan，Lu,Gao，Shu Hong,et al. Strain-level multidrug-resistant pathogenic bacteria in urban wastewater treatment plants: Transmission, source tracking and evolution[J]. Water Research,2024,267.

Zhao，Yanmei.,Fan，Lu.,Gao，Shu Hong.,Huang，Fang.,Lei，Zelin.,...&Wang，Aijie.(2024).Strain-level multidrug-resistant pathogenic bacteria in urban wastewater treatment plants: Transmission, source tracking and evolution.Water Research,267.

Zhao，Yanmei,et al."Strain-level multidrug-resistant pathogenic bacteria in urban wastewater treatment plants: Transmission, source tracking and evolution".Water Research 267(2024).