On-chip three-dimensional imaging and counting of particles transported in a microfluidic channel based on light-field microscopy

Xinglong Huang1,2; Xing Cheng2,3; Boris Stoeber4

2024

10.1109/JSEN.2024.3466172

IEEE Sensors Journal   影响因子和分区

2379-9153

Rapid acquisition of 3D volumetric information and accurate counting are significant in observing and monitoring specimens moving in microfluidic channels. This study introduces a novel approach of 3D imaging flow cytometry (IFC) using light-field microscopy that images the microfluidic channel with a microlens array (MLA) and reconstructs a complete 3D volume from a single frame. To create the custom MLA, a cost-effective method is developed, combining gray-scale photolithography, nickel electroplating, and nanoimprinting. Furthermore, a calibration procedure is devised to rectify aberrations within the imaging system prior to 3D reconstruction. The counting process employs an algorithm based on local intensity peak identification to quantify and locate particles moving in a microchannel, achieving counting accuracies around 95%. In comparison to existing 3D IFCs, our method offers distinct advantages, including the capability for high throughput data capture and 3D imaging fast moving samples without necessitating complex microchannel designs.

其他

1.Department of Mechanical Engineering, University of British Columbia, Vancouver, CA, USA
2.Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, China
3.SUSTech-Jiaxing Research Center, Jiaxing, China
4.Department of Mechanical Engineering and the Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, CA, USA

Xinglong Huang,Xing Cheng,Boris Stoeber. On-chip three-dimensional imaging and counting of particles transported in a microfluidic channel based on light-field microscopy[J]. IEEE Sensors Journal,2024,PP(99).

Xinglong Huang,Xing Cheng,&Boris Stoeber.(2024).On-chip three-dimensional imaging and counting of particles transported in a microfluidic channel based on light-field microscopy.IEEE Sensors Journal,PP(99).

Xinglong Huang,et al."On-chip three-dimensional imaging and counting of particles transported in a microfluidic channel based on light-field microscopy".IEEE Sensors Journal PP.99(2024).