Multiplexed Random Access Approach to DNA Microspheres for High-Capacity Data Storage

Zhong, Wukun1; Wang, Saijie2; Geng, Chunyang2; Zheng, Yanlin1; Bai, Shiyan1; Cao, Xiuping1; Liu, Kai3; Yang, Yang4,5; Lu, Chunhua1; Jiang, Xingyu2

2024-10-01

10.1002/adfm.202408852

ADVANCED FUNCTIONAL MATERIALS   影响因子和分区

1616-301X

1616-3028

["With the rapid growth of modern information data, DNA has emerged as a novel medium for data storage due to its durability, replicability, sustainability, and high density. This study focuses on the compatibility, high capacity, and random access of DNA data storage systems. A calcium phosphate (CaP) mineralized fluorescent SiO2 DNA (FSD@CaP) microspheres is developed by encapsulating fluorescence-labeled encoding DNA files onto the surface of the SiO2 microspheres that are intrinsically sized and fluorescent as addressed two-dimensional (2D) barcodes. The various sizes of SiO2 and various fluorescence-labeled encoding DNA files forming 2D barcodes of FSD@CaP. The 2D barcodes enhance the system's compatibility while allowing multiplexed random access to DNA files. The high-capacity DNA storage permits effective single access to the DNA file using a minimal number of FSD@CaP microspheres, without impacting the matrix of the system. Ultimately, random access to arbitrary DNA files within a complex pool of distinct 2D barcode-tagged FSD@CaP microspheres is demonstrated by utilizing a flow cytometer for multiplexed sorting. Consequently, the strategy of this storage system provides a scalable concept for compatibility and multiplexed random access to DNA data sets.","The FSD@CaP microspheres encapsulate encoding DNA file sequences with fluorescence within mineralized microspheres that are intrinsically sized and fluorescent as addressed in 2D barcodes. By sorting different 2D FSD@CaP via flow cytometry, the acquisition of the corresponding DNA file is enabled, thereby achieving random access to the DNA file. While non-target DNA files can be recycled for further use. image"]

DNA data storage system microfluidic chip integration multiplexed random access randomized non-destructive access

SCI

英语

其他

National Natural Science Foundation of China[22174019] ; null[2018YFA0902600]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:001326634500001

WILEY-V C H VERLAG GMBH

Web of Science

1.Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350108, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China
3.Tsinghua Univ, Engn Res Ctr Adv Rare Earth Mat, Dept Chem, Minist Educ, Beijing 100084, Peoples R China
4.Shanghai Jiao Tong Univ, Inst Mol Med, Shanghai 200127, Peoples R China
5.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Shanghai Key Lab Nucl Acid Chem & Nanomed,State Ke, Shanghai 200127, Peoples R China

Zhong, Wukun,Wang, Saijie,Geng, Chunyang,et al. Multiplexed Random Access Approach to DNA Microspheres for High-Capacity Data Storage[J]. ADVANCED FUNCTIONAL MATERIALS,2024.

Zhong, Wukun.,Wang, Saijie.,Geng, Chunyang.,Zheng, Yanlin.,Bai, Shiyan.,...&Jiang, Xingyu.(2024).Multiplexed Random Access Approach to DNA Microspheres for High-Capacity Data Storage.ADVANCED FUNCTIONAL MATERIALS.

Zhong, Wukun,et al."Multiplexed Random Access Approach to DNA Microspheres for High-Capacity Data Storage".ADVANCED FUNCTIONAL MATERIALS (2024).