Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing

Pereira, Olivier1,2; Qin, Wei3; Galand, Pierre E.4; Debroas, Didier5; Lami, Raphael6; Hochart, Corentin4; Zhou, Yangkai1; Zhou, Jin7; Zhang, Chuanlun1,8

2024-09-01

10.1002/mlf2.12144

MLIFE   影响因子和分区

2097-1699

2770-100X

Ammonia-oxidizing archaea (AOA) play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification. Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA. Increasing studies suggest that quorum sensing (QS) mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes; however, this has yet to be demonstrated in marine planktonic archaea. To bridge this knowledge gap, we employed a combination of metabolic activity markers (amoA, accA, and grs) to elucidate the regulation of AOA-mediated nitrogen, carbon processes, and their interactions with the surrounding heterotrophic population. Through co-transcription investigations linking metabolic markers to potential key QS genes, we discovered that QS molecules could regulate AOA's carbon, nitrogen, and lipid metabolisms under different conditions. Interestingly, specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population. Overall, our data demonstrate that QS orchestrates nitrogen and carbon metabolism, including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria, which has been previously overlooked in marine AOA research.

AOA functional genes marine ammonia-oxidizing archaea networking quorum sensing Tara Oceans

ESCI

英语

第一 ; 通讯

Tara Oceans consortium["42321004","92351301"] ; National Natural Science Foundation of China[20200925173954005] ; Stable Support Plan Program of Shenzhen Natural Science Fund[KCXFZ20211020174803005] ; Shenzhen Municipal Commission of Science and Technology Innovation["2021B1515120080","2020B1515120012"] ; Guangdong Basic and Applied Basic Research Foundation[ZDSYS201802081843490] ; Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Southern University of Science and Technology[ANR-14-CE02-0004-01] ; Science Plan of the UN Ocean Decade Global Ocean Negative Carbon Emissions (Global ONCE) Program[518055]

Microbiology

Microbiology

WOS:001327101400007

WILEY

Web of Science

1.Southern Univ Sci & Technol, Dept Sci & Engn, Shenzhen Key Lab Marine Geoom Archaea, Shenzhen, Peoples R China
2.Wuhan Univ Technol & Aix Marseille Universite?, Inst WUT AMU, Wuhan, Peoples R China
3.Univ Oklahoma, Inst Environm Genom, Sch Biol Sci, Norman, OK USA
4.Sorbonne Univ, CNRS, Lab Ecogeochim Environm Benth LECOB, Banyuls Sur Mer, France
5.Univ Clermont Auvergne, CNRS, Lab Microorganismes Genome & Environm, Clermont Ferrand, France
6.Sorbonne Univ, CNRS, Lab Biodivers & Biotechnol Microbiennes LBBM, Banyuls Sur Mer, France
7.Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen Publ Platform Screening & Applicat Marine, Shenzhen, Peoples R China
8.Shanghai Earthquake Agcy, Shanghai Sheshan Natl Geophys Observ, Shanghai, Peoples R China

Pereira, Olivier,Qin, Wei,Galand, Pierre E.,et al. Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing[J]. MLIFE,2024,3(3).

Pereira, Olivier.,Qin, Wei.,Galand, Pierre E..,Debroas, Didier.,Lami, Raphael.,...&Zhang, Chuanlun.(2024).Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing.MLIFE,3(3).

Pereira, Olivier,et al."Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing".MLIFE 3.3(2024).